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Horm Metab Res 1971; 3(1): 10-20
DOI: 10.1055/s-0028-1095040
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© Georg Thieme Verlag KG Stuttgart · New York

Effect of Diabetes or Hyperglycemia on Lipid Metabolism and Urea Formation by the Perfused Rat Liver[*]

J. C. Penhos [**] , J. C. Basabe , N.  Lopez , R.  Levine
  • Veterans Administration Hospital Washington, D.C., USA
    Department of Medicine, George Washington University, Washington, D.C., USA
    Research Foundation, Washington Hospital Center, Washington, D.C., USA
    Department of Medicine, New York Medical Center, New York City, USA
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Publication History

Publication Date:
07 January 2009 (online)

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Abstract

The influence of high blood sugar levels on lipid metabolism and urea production in the "diabetic liver" (DL) have been studied in isolated rat liver perfusion.

For comparative purposes DL were perfused with normoglycemic blood plus hyperlipemic serum (HLS) while "normal" livers (NL) were perfused with hyperglycemic blood and HLS. Diabetic livers were obtained from subtotally pancreatectomized rats (250-300 mg per 100 ml blood sugar). In DL perfusions, addition of HLS increased the blood sugar level and ketone body (KB) production while addition of glucose reduced the increased KB formation by HLS in NL perfusions. When HLS was added to both groups of perfusions, there was a transitory rise followed by a decrease in perfusate levels of FFA and triglycerides that were more marked in DL. An increase in the liver triglycerides and cholesterol also occurred in both types of perfusions, although the rise was higher in DL. Glycogen increased in both groups of perfusions but was significantly higher in NL.Urea formation was increased in DL perfusions with or without addition of HLS. In NL perfusions, the urea levels were raised when HLS was added to "starved" perfusions and was significantly reduced by simultaneous addition of glucose.

Thus in the presence of elevated lipemic levels, high blood sugar concentrations play an important role in lipid metabolism, KB production and urea formation in NL but not in DL.

Key words

Perfused Rat Liver - Lipid Metabolism - Urea Formation - Hyperglycemia - Glycogen

1 These studies were partially supported by NIH Grant (AM 13101-01)

1 These studies were partially supported by NIH Grant (AM 13101-01)

2 Present address: Endocrine Research Veterans Administration Hospital, Washington, D.C., 20422.

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