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Planta Med 2012; 78(16): 1787-1795
DOI: 10.1055/s-0032-1315371
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

LC-MS N-alkylamide Profiling of an Ethanolic Anacyclus pyrethrum Root Extract

Jente Boonen
1   Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium
,
Vikas Sharma
2   Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar M. P., India
,
Vinod Kumar Dixit
2   Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar M. P., India
,
Christian Burvenich
3   Department of Physiology and Biometrics, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
,
Bart De Spiegeleer
1   Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium
› Author Affiliations
Further Information

Publication History

received 04 June 2012

accepted 22 August 2012

Publication Date:
09 October 2012 (online)

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Abstract

An N-alkylamide profiling from an ethanolic Anacyclus pyrethrum DC. root extract was performed using a gradient reversed phase high performance liquid chromatography/UV/electrospray-ionization ion-trap mass spectrometry (HPLC/UV/ESI-MS) method on an embedded polar column. MS1 and MS2 fragmentation data were used for identification purposes while UV was used for quantification. Thirteen N-alkylamides (five N-isobutylamides, three N-methyl isobutylamides, four tyramides and one 2-phenylethylamide) were detected. Six of them, identified as undeca-2E,4E-diene-8,10-diynoic acid isobutylamide, undeca-2E,4E-diene-8,10-diynoic acid N-methyl isobutylamide, tetradeca-2E,4E-diene-8,10-diynoic acid tyramide, deca-2E,4E-dienoic acid N-methyl isobutylamide, tetradeca-2E,4E,XE/Z-trienoic acid tyramide and tetradeca-2E,4E,XE/Z,YE/Z-tetraenoic isobutylamide, are novel compounds which have never been reported before from Anacyclus pyrethrum.

Key words

Anacyclus pyrethrum - Asteraceae - LC-MS plant profiling - N-alkylamides - pellitorine - anacycline
 

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