Influence of Cell Age and Ketoaminic Linkage on Rapid Glycosylation of Hemoglobin in Human Red Cells In Vitro

Summary

In vitro the rate of synthesis of the aldiminic linkage between Hb and glucose depends on glucose concentration, length of incubation and some other physiological factors.

To understand better the regulation of this synthesis and to verify the role of cell age and of basal HbA₁ levels on the rate of synthesis of pre-A₁ we studied red cells from 7 normal controls and 7 diabetics, with high HbA₁ levels.

We found that the content of HbA₁ (stable glycosylated hemoglobin) is able to negatively affect the rate of synthesis of new pre-A₁, according to a curvilinear model.

These results suggest that in vitro the glycosylation process is saturable, and that elevated values of HbA₁ are able to slow the synthesis of pre-A₁ in vitro.

Key-Words:

Glycosylated Hemoglobin - Diabetes Mellitus - Red Cell Age - Rapid Glycosylation