Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596919
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Analysis on bioavailability and metabolite profile of anti-hyperuricemic fraction of alfalfa extract in a cell co-culture model coupled with UPLC-ESI-Q-TOF-MS/MS

SJ Hsu
1   Department of Food Science, National Chiayi University, No.300 Syuefu Rd., Chiayi 60004, Taiwan (R.O.C.)
,
SM Lin
1   Department of Food Science, National Chiayi University, No.300 Syuefu Rd., Chiayi 60004, Taiwan (R.O.C.)
,
CK Lee
2   School of Pharmacy, Taipei Medical University, No. 250 Wu Xin St.Taipei 11031, Taiwa (R.O.C.) e-mail:cklee@tmu.edu.tw
,
HL Kuo
3   Department of Food Nutrition, Chung Hwa University of Medical Technology, No.89, Wenhwa 1st St., Rende Shiang, Tainan County 717, Taiwan (R.O.C.)
,
CH Chang
1   Department of Food Science, National Chiayi University, No.300 Syuefu Rd., Chiayi 60004, Taiwan (R.O.C.)
› Author Affiliations
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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    Medicago sativa Linn. (Leguminosae) is commonly known as Alfalfa which means “father of all foods”. Alfalfa is not only a good source of animal feed but also a therapeutic agent used in anecdotal medicine. It has been demonstrated to contain various active compounds that are beneficial for human health [1]. Recent pharmacological studies show that alfalfa extract exhibits neuroprotective, hypocholesterolemic, antioxidant, antiulcer, antimicrobial, hypolipidemic, and estrogenic activity, and is effective for treating atherosclerosis, heart disease, stroke, cancer, diabetes and menopausal symptoms. We found that alfalfa leaves have anti-hyperuricemic potential because of its xanthine oxidase inhibitory activity. By using biochemical analysis in conjunction with liquid chromatography tandem mass spectrometry (HPLC-DAD-ESI-MS) technique, we verified that the anti-hyperuricemic fraction of alfalfa extract contain various functional ingredients, including salicylic acid, sinapic acid, p-coumaric acid, apigenin, tricin, and chrysoeriol. The anti-hyperuricemic fraction was further subjected for the examination of bioavailability and metabolite profile produced by intestine cells and hepatocytes in a transwell cell co-culture model in conjunction with UPLC-ESI-Q-TOF-MS/MS analysis. Anti-hyperuricemic fraction of alfalfa extract was included in the medium of the upper layer in the cell culture model comprising Caco-2 cells in upper layer (transwell) and HepG2 cell in bottom layer. The cell medium of the both layers were collected seperately at different time points after the treatment, followed by UPLC-ESI-Q-TOF-MS/MS analysis to detect the distribution of functional components and varify the active compounds as well as the corresponding metabolite profile generated by intestinal and hepatic metabolism. The established model can also be useful for rapid evaluation of bioavailability, metabolite profile and hepatic protection activity of orally administered natural products.

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    Keywords: UPLC-ESI-QTOF-MS/MS, co-culture, bioavailability.

    References:

    [1] Bora KS, Anupam S. Phytochemical and pharmacological potential of Medicago sativa: A review. Pharm Biol 2011; 49: 211 – 220


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    No conflict of interest has been declared by the author(s).

     
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