Subscribe to RSS
DOI: 10.1055/s-0038-1641043
Cell viability of vocal fold implants constructed by magnetic tissue engineering
Introduction:
Voice disorders cause a severe reduction of quality of life. Especially defects of the vocal folds are of importance; e.g., after tumour surgery. There are no implants in the field of phono surgery yet, which can be used as a therapeutic tool. By means of magnetic tissue engineering, we created a three-dimensional structure, which now has to be tested and optimized.
Methods:
Rabbit vocal fold fibroblasts have been incubated with superparamagnetic iron oxide nanoparticles in a concentration of 20 µg/cm2. Subsequently, variable configured vocal fold implants were designed by using different magnets. Cell viability was analysed in flow cytometry by staining with annexin V/propidium iodide. Additionally, we performed an immunohistochemical examination with phalloidine (cytoskeleton) and Hoechst 33342 (nucleus).
Results:
We detected not less than 80% cell viability in terms of the different forms. Apoptotic (annexin V positive) and necrotic cells (propidium iodide positive) were equally distributed. Regarding the immunhistochemical staining we found a dense cell structure in the implants.
Conclusion:
We demonstrated the wide viability of our vocal fold implants constructed by using magnetic tissue engineering. These results take us a step forward in the development of vocal fold implants.
#
No conflict of interest has been declared by the author(s).
Publication History
Publication Date:
18 April 2018 (online)
© 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Georg Thieme Verlag KG
Stuttgart · New York