CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S385
DOI: 10.1055/s-0038-1641051
Abstracts
Tissue Engineering/Stammzellen: Tissue Engineering/Stem Cells

The influence of a static magnetic field on co-cultures of mesenchymal stem cells and human satellite cells

CE Müller
1   HNO-Universitätsklinik Mannheim, Mannheim
,
J Stern-Sträter
1   HNO-Universitätsklinik Mannheim, Mannheim
,
N Rotter
1   HNO-Universitätsklinik Mannheim, Mannheim
,
R Birk
2   HNO-Universitätsklinikum Marburg, Marburg
,
K Hörmann
1   HNO-Universitätsklinik Mannheim, Mannheim
,
C Weilbach
3   Klinik für Anästhesiologie und Intensivmedizin, St. Josefs Krankenhaus, Cloppenburg
› Author Affiliations
› Further Information
Also available at
 
 

    Background:

    The results of surgical repair of tissue defects are still less than ideal leaving patients with residual cosmetic and functional impairments. Therefore, tissue engineering attempts to grow functional neo-tissue to support defect closure. The goal to form functional skeletal muscle using clinically feasible stimuli has not yet been reached to a sufficient extent. This study investigated the differentiation potential of static magnetic fields (SMF), using co-cultures of human satellite cells and human mesenchymal stem cells (MSC) which have been found to be promising in animal trials.

    Material and Methods:

    Tests were performed on co-cultures with and without SMF exposure, using growth (high growth factor concentrations [GM]) and differentiation medium (low growth factors concentrations [DM]) over 12 days. The SMF was produced by a neodymiummagnet (4 × 4 cm) with a strength of 80 ± 5 mT placed beneath the cultures. The proliferation analysis was measured by an alamarBlue® assay.

    Semi-quantitative gene expression measurements of the following marker genes showed the degree of differentiation: myogenic factor 5 (MYF5), myogenic differentiation antigen 1 (MYOD1), myogenin, adult myosin heavy chain (MYH1), and skeletal muscle α1 actin (ACTA1).

    Results:

    Although an upregulation of MYOD1, Myogenin and ACTA1 could be found in DM, neither in GM nor in DM a significant increase in marker gene expression could be detected which was confirmed by a missing immunhistochemical antibody staining.

    Conclusion:

    SMF exposure did not enhance myogenic maturation as no rise in differentiation markers was observed and did not result in the desired increase in myogenic differentiation. Further studies are needed to identify a suitable stimulus for skeletal muscle tissue engineering.


    #

    No conflict of interest has been declared by the author(s).

    C. Emika Müller
    HNO-Universitätsklinik Mannheim,
    Theodor-Kutzer-Ufer 1 – 3, 68161,
    Mannheim

    Publication History

    Publication Date:
    18 April 2018 (online)

    © 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

    Georg Thieme Verlag KG
    Stuttgart · New York