CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S199-S200
DOI: 10.1055/s-0039-1686885
Abstracts
Tissue Engineering/Stem Cells

The role of thrombospondin-1 in biomaterial integration of porous polyethylene implants in vivo

J Eckrich
1   Universitätsmedizin Mainz, HNO Klinik, Mainz
,
A Maas
1   Universitätsmedizin Mainz, HNO Klinik, Mainz
,
K Jurk
2   Zentrum für Thrombose und Hämostase der Universitätsmedizin der Johannes Gutenberg-Universität Mainz, Mainz
,
S Strieth
1   Universitätsmedizin Mainz, HNO Klinik, Mainz
,
E Kumm
2   Zentrum für Thrombose und Hämostase der Universitätsmedizin der Johannes Gutenberg-Universität Mainz, Mainz
,
J Brieger
1   Universitätsmedizin Mainz, HNO Klinik, Mainz
› Author Affiliations
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    Introduction:

    Thrombospondin-1 (TSP1) is a matrix glycoprotein with functions in angiogenesis and thrombogenesis. The aim of this study was to investigate the role of TSP-1 for angiogenesis and host tissue integration of porous polyethylene (PPE) implants used for reconstruction of bone and cartilaginous tissue in craniomaxillofacial surgery.

    Methods:

    Over a period of ten days, functional vessel density, cellular immune response as well as platelet-endothelial cell interactions were examined by fluorescence microscopy after implantation of PPE into dorsal skinfold chambers in TSP-1 knock-out mice (TSP-1 –/-) [n = 8] and wild-type mice [n = 8].

    Results:

    In the group of TSP –/– mice a significantly reduced functional vessel density within the implanted PPE was found. Furthermore, the vessel network in the tissue surrounding the implant also showed signs of an impaired vascular network. Leukocyte adherence and platelet-endothelial cell interactions also showed differences in both groups.

    Conclusions:

    TSP-1 has been shown to influence neovascularization and integration of PPE implants into host tissue. Therefore, TSP-1 coating represents a new promising approach for pro-angiogenic surface modification of implant surfaces for plastic-reconstructive procedures.


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    Dr. med. Jonas Eckrich
    Universitätsmedizin Mainz, HNO Klinik,
    Langenbeckstraße 1, 55131
    Mainz

    Publication History

    Publication Date:
    23 April 2019 (online)

    © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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