Zeitschrift für Phytotherapie 2019; 40(S 01): S39-S40
DOI: 10.1055/s-0039-1697324
Poster
Georg Thieme Verlag KG Stuttgart · New York

Stress-induced changes in intestinal permeability in mice: Influence of STW 5

P Aubert
1   Inserm U1235-Institut des Maladies de l'Appareil Digestif du CHU de Nantes, Nantes, France
,
J Chevalier
1   Inserm U1235-Institut des Maladies de l'Appareil Digestif du CHU de Nantes, Nantes, France
,
T Durand
1   Inserm U1235-Institut des Maladies de l'Appareil Digestif du CHU de Nantes, Nantes, France
,
A Bessard
1   Inserm U1235-Institut des Maladies de l'Appareil Digestif du CHU de Nantes, Nantes, France
,
O Kelber
2   Research & Development, Bayer Consumer Health, Steigerwald Arzneimittelwerk GmbH, Darmstadt, Germany
,
H Aziz-Kalbhenn
2   Research & Development, Bayer Consumer Health, Steigerwald Arzneimittelwerk GmbH, Darmstadt, Germany
,
M Neunlist
1   Inserm U1235-Institut des Maladies de l'Appareil Digestif du CHU de Nantes, Nantes, France
› Author Affiliations
Further Information

Publication History

Publication Date:
09 September 2019 (online)

 
 

    The herbal preparation STW 5 has recently been reported to increase intestinal chloride secretion. However, its ability to modulate paracellular and transcellular permeability remains currently unknown. Therefore, we aimed to study the ability of STW 5 to modulate intestinal permeability under basal and repeated acute stress conditions.

    C57 bl6 mice were gavaged for 14 days with STW 5 (3 ml/kg). After 10 days of treatment, mice were subjected to water avoidance stress (WAS) during 4 consecutive days. In vivo permeability to FITC -Sulfonic Acid (FSA, 400 Da) and Horse Radish Peroxydase (HRP, 44KDa), total transit time and colonic transit (fecal pellet output – FPO) were assessed at Day 0 (D0), D10 and D14 of IB treatment. Ex vivo permeability to FSA and HRP was assessed on jejunum, ileum, proximal colon and distal colon at D14 using Ussing chambers. Tight junction protein expression was assessed in colon tissue using RT-PCR and Western Blot analysis.

    In vivo permeability to FSA and HRP as well as total transit time were not modified by STW 5 in basal and WAS conditions. However, STW5 prevented the increase in permeability to FSA induced by WAS in the distal colon ex vivo. Conversely, STW 5 prevented the increase in permeability to HRP induced by WAS in the jejunum and proximal colon. Furthermore, while STW 5 tended to increase colonic transit as compared to control in basal conditions, it prevented the increase in colonic transit induced by WAS. In addition, STW 5 tended to increase expression of the tight junction proteins JAMA, ZO-1 and cingulin as reflected by Western Blots.

    Our study suggests that STW 5 can prevent WAS induced changes in paracellular and transcellular permeability in specific regions of the gastrointestinal tract. Such effects could contribute to the therapeutic effects of STW 5 in irritable bowel syndrome and support novel therapeutic indications for pathologies in which barrier functions are altered.

    Acknowledgement:

    The study was supported by Steigerwald Arzneimittelwerk GmbH.


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