Validated HPLC-DAD method for resveratrol determination during identification in raw materials and stability, preformulation, permeability studies

Resveratrol (3,4′,5- trihydroxystilbene) is a nutraceutical that has exerted anti-inflammatory and antioxidant activity [1]. It has been found also as anticancer agent, and substance with effect on neurological diseases and other maladies[2].

The aim of this study was to develop an HPLC-DAD method suitable for resveratrol determination during identification in raw materials and its stability, preformulation and permeability studies.

The HPLC method with UV detection with the Kinetex C18 column (100 × 2.1 mm, 5 μm) was used. The mobile phase consisted of ACN/0.5% acetic acid in ration 20/80. The detection wavelength was 306 nm, the column temperature was set at 40°C. The method meets all required validation parameters (selectivity, linearity, precision, accuracy). The changes of resveratrol permeability was investigated by using PAMPA test.

The proposed RP-HPLC method is a suitable technique for the determination of resveratrol in different approaches such as preformulation, stability and permeability studies. Resveratrol permeability coefficient (pH 7.4) was 74.89±27.28x10^-6 cm/s, what classified drug as well-permeable.

Acknowledgment

The part of the research was funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 778051 and the Ministry of Science and Higher Education of Poland fund for supporting internationally co-financed projects in 2018–2022 (agreement No 3899/H2020/2018/2).

• References

• 1 Gülçin İ. Antioxidant properties of resveratrol: A structure–activity insight. Innovative Food Sci Emerg Technol 2010; 11: 210-218.
• 2 Berman AY, Motechin RA, Wiesenfeld MY, Holz MK. The therapeutic potential of resveratrol: a review of clinical trials. NPJ Precis Oncol. 2017 1. .