Planta Med 2019; 85(18): 1461-1462
DOI: 10.1055/s-0039-3399828
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Efficient isolation of new bioactive metabolites from the marine endophytic fungi Fusarium solani

A Alfattani
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne,, CH-1206 Geneva, Switzerland
,
EF Ferreira Queiroz
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne,, CH-1206 Geneva, Switzerland
,
L Marcourt
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne,, CH-1206 Geneva, Switzerland
,
S Leoni
2   Microbiology Unit, University of Geneva,, Department of Botany and Plant Biology, Geneva, Switzerland
,
P-M Allard
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne,, CH-1206 Geneva, Switzerland
,
K Perron
2   Microbiology Unit, University of Geneva,, Department of Botany and Plant Biology, Geneva, Switzerland
,
D Stien
3   Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique,, Banyuls-sur-Mer, France
,
K Gindro
4   Agroscope, Plant Protection Research Division,, Route de Duillier 50, P.O. Box 1012, 1260 Nyon, Switzerland
,
J-L Wolfender
1   School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne,, CH-1206 Geneva, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Endophytes are organisms, often fungi and bacteria that live between plant cells. The relationship they establish with the plant varies from symbiotic to almost pathogenic. Endophytes have proven to be a promising source of bioactive natural products with interesting structural diversity. In the present study, the secondary metabolites of the endophytic fungi Fusarium solani was investigated. This fungus was isolated from the rhizomes of the marine herbaceous plant Posedonia oceanica harvested in the Mediterranean Sea. The ethyl acetate extract of F. solani presented antimicrobial activities against Staphylococcus aureus and Pseudomonas aeruginosa. In order to isolate the compounds responsible for these activities, the culture of F. solani was up-scaled and purified by semi-prep. HPLC. A new procedure based on dry charge injection was used in order to maintain the column resolution over high charges. This approach resulted in the successfully one-step isolation of 23 compounds, 16 of them are original natural products possessing interesting antimicrobial activities. known compounds were dereplicated by in silico databases search based on UHPLC-HRMS. A molecular network of structure similarity was created within the extract based on MS-MS spectra. The structure elucidation of the isolated compounds was performed based on NMR and HRMS data analysis.


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  • References

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  • References

  • 1 Nisa H, Kamili AN, Nawchoo IA, Shafi S, Shameem N, Bandh SA. Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: A review. Microb Pathogenesis 2015; 82: 50-59.
    CrossrefPubMedGoogle Scholar
  • 2 Singh M, Kumar A, Singh R, Pandey KD. Endophytic bacteria: a new source of bioactive compounds. 3 Biotech. 2017 7: DOI: 10.1007/s13205-13017-10942-z
    CrossrefPubMedGoogle Scholar
  • 3 Queiroz EF, Alfattania A, Afzana A, Marcourt L, Guillarme D, Wolfender J-L. Utility of dry load injection for an efficient natural products isolation at the semi-preparative chromatographic scale. J Chromatogr A. 2019 In press:.
    PubMedGoogle Scholar