CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S38
DOI: 10.1055/s-0040-1710834
Abstracts
Allergology/Environmental Medicine/Immunology

Stimulation of isolated CD4+ and CD8+ T cells with Candida albicans in patients with chronic rhinosinusitis (CRS)

P Ickrath
1   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Universitätsklinik Würzburg Würzburg
,
L Sprügel
1   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Universitätsklinik Würzburg Würzburg
,
N Beyersdorf
2   Institut für Virologie und Immunbiologie, Universität Würzburg Würzburg
,
R Hagen
1   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Universitätsklinik Würzburg Würzburg
,
S Hackenberg
1   Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Universitätsklinik Würzburg Würzburg
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    Bacteria and fungi can act as superantigens regarding T cell activation. Previous studies have shown a specific stimulation of CD4+ T cells in patients with CRSwNP. The influence of C. albicans on the pathogenesis of CRS is discussed controversially and its exact role is not clear up to date. The aim of the present study was to measure the influence of C. albicans on the activation of CD4+ and CD8+ T cells in patients with CRS with and without nasal polyps.

    Tissue and blood samples were collected from 8 patients suffering from chronic rhinosinusitis with and without nasal polyps (n = 10) undergoing nasal sinus surgery respectively. Furthermore, antigen-presenting cells (APC) were obtained from peripheral blood. CD4+ and CD8+ cells were cultured together with APC in each case. A peptide pool with C. albicans was added to tissue and blood samples for 6 days. After 6 days, lymphocytes were separated and analyzed by multicolor flow cytometry. Activation was assessed by the intracellular marker Ki-67 and the cytokine secretion was measured in the supernatant.

    No statistical differences on Ki67 expression of CD4+ and CD8+ T cells after stimulation by C. albicans were seen between peripheral blood lymphocytes and tissue samples in patients with CRSwNP or CRSsNP as well as in the activation of tissue CD4+ or CD8+ T cells in CRSwNP compared to CRSsNP. No differences were measured between tissue CD8+ T cells of CRSwNP compared to CRSsNP and cytokine secretion was similar between the study groups.

    In this study, no specific stimulation of CD4+ and CD8+ T cells by C. albicans was seen. C. albicans does not seem to play any role in the pathogenesis of CRS with and without nasal polyps despite its high local presence.

    Poster-PDF A-1152.PDF


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    Dr. med. Pascal Ickrath
    Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen, Universitätsklinik Würzburg
    Josef-Schneider-Strasse 11
    97082 Würzburg

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    Artikel online veröffentlicht:
    10. Juni 2020

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