CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S147-S148
DOI: 10.1055/s-0040-1710976
Abstracts
Oncology

HPV status as predictive biomarker in head and neck cancer – which method fits the best for outcome prediction?

M Linxweiler
1   Univ. HNO-Klinik Homburg/S.
,
W Schmid
1   Univ. HNO-Klinik Homburg/S.
,
S Körner
1   Univ. HNO-Klinik Homburg/S.
,
F Bochen
1   Univ. HNO-Klinik Homburg/S.
,
S Wemmert
1   Univ. HNO-Klinik Homburg/S.
,
S Smola
2   Universitätsklinikum des Saarlandes, Institut für Virologie Homburg/S.
,
S Lohse
2   Universitätsklinikum des Saarlandes, Institut für Virologie Homburg/S.
,
M Wagner
3   Universitätsklinikum des Saarlandes, Institut für Allgemeine und Spezielle Pathologie Homburg/S.
,
B Schick
1   Univ. HNO-Klinik Homburg/S.
› Author Affiliations
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    Introduction Head and neck squamous cell carcinomas (HNSCCs) belong to the six most commen cancers worlwide and are associated with a poor prognosis. Over the past years, infection of the oral mucosa with high risk human papillomavirus (HPV) was discovered as an additional risk factor apart from well-known carcinogenic effects of chronic tobacco and alcohol consumption. In clinical studies, HPV-induced HNSCCs showed a better response to radiation and radiochemotherapy compared with tobacco and alcohol induced cancers. However, there is no standardized method for determining HPV status in HNSCCs, so far.

    Methods Tissue samples from 153 HNSCC patients with a median follow-up of 46,28 months (15 – 186 months) were analyzed with HPV-DNA-in situ hybridization (HPV-DNA-ISH), HPV-DNA-PCR and immunohistochemistry (IHC) targeting p16, p16 and Ki67, pRB or Cyclin D1 and analyzed for their relevance as prognostic biomarkers.

    Results Positive HPV-DNA-ISH (p = 0.0039), positive HPV-DNA-PCR (p = 0.0113), positive p16-Ki67-IHC (p = 0.0047) and positive p16-IHC (p = 0.003) showed a significant correlation with the patients’ overall survival. Cyclin D1 and pRb expression had no prognostic relevance in our analysis. The prognostic validity was not further enhanced by combining the analyzed biomarkers.

    Conclusion P16 IHC showed the best correlation with the patients‘ overall survival with nearly equal prognostic validity for p16-Ki67-IHC and HPV-DNA-ISH. For the future, a worldwide standard for HPV diagnostics in head and neck oncology should be pursued.

    Poster-PDF A-1038.pdf


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    Priv.-Doz. Dr. med. Linxweiler Maximilian
    Univ. HNO-Klinik
    Kirrbergerstraße 100, Gebäude 6
    66421 Homburg/S.

    Publication History

    Article published online:
    10 June 2020

    © 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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