Fiber-based neural guidance scaffold

Introduction Current approaches to improve implant function are concentrating on the optimization of the nerve-electrode-interface. Poly-e-caprolactone (PCL) and polyglycolide (PGA) have already shown great potential as an artificial guideance structure for neurons on the surface of the implant. The aim of the study is to use PCL- and PGA-fibres in cochlear implants as local drug delivery system for neurotrophic factors.

Method Polymer fibers were functionalized by aminolysis with ethylenediamine with amino groups. Subsequently, heparan sulfate (HS) was covalently bound to the fibers and the neurotrophic factor (BDNF) was immobilized on the surface. The amino-functionalization of the fiber was examined microscopically by fluorescence labeling (FITC) and the binding of HS was checked indirectly via a toluidine blue assay. The immobilization of BDNF was analyzed by release experiments. The concentration of BDNF was determined by ELISA and the activity of BDNF was examined in cell culture investigations with spiral ganglion neurons (SGN).

Results The successful functionalization and the binding of HS to the polymer fibers could be confirmed. Cell culture investigations showed an increased survival of SGN and confirmed the bioactivity of the released BDNF. Over a period of 100 days BDNF was released.

Conclusion The first step in the development of a fiber-based neural guidance scaffold is completed by the successful functionalization of the fiber. Further investigations must now show the directed growth of the neurons at the guidance scaffolds.

Poster-PDF A-1628.PDF

Cluster of Excellence Hearing4all

Publication History

Article published online:
10 June 2020