Z Gastroenterol 2021; 59(01): e11
DOI: 10.1055/s-0040-1721971
Poster Visit Session I Basic Hepatology (Fibrogenesis, NPC, Transport)
Friday, January 29, 2021, 12:30 pm – 1:15 pm, Poster Session Virtual Venue

Schistosoma mansoni eggs modulate the host´s hepatic lipid metabolism

L Hehr
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
,
V von Bülow
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
,
S Gindner
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
,
N Buß
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
,
A Baier
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
,
G Schramm
2   Research Center Borstel, Focus on Asthma & Allergy, Borstel, Germany
,
T Quack
3   Justus Liebig University, Institute for Parasitology, Giessen, Germany
,
CG Grevelding
3   Justus Liebig University, Institute for Parasitology, Giessen, Germany
,
S Gerbig
4   Justus Liebig University, Institute of Inorganic and Analytical Chemistry, Giessen, Germany
,
P Kadesch
4   Justus Liebig University, Institute of Inorganic and Analytical Chemistry, Giessen, Germany
,
Ventura A Peter
4   Justus Liebig University, Institute of Inorganic and Analytical Chemistry, Giessen, Germany
,
KR Wiedemann
4   Justus Liebig University, Institute of Inorganic and Analytical Chemistry, Giessen, Germany
,
B Spengler
4   Justus Liebig University, Institute of Inorganic and Analytical Chemistry, Giessen, Germany
,
M Roderfeld
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
,
E Roeb
1   Medical Clinic II/Justus Liebig University, Gastroenterology, Giessen, Germany
› Author Affiliations
 
 

    Questions Schistosomiasis (formerly Bilharzia) is a parasitic infectious disease provoked by Schistosoma mansoni. The disease is endemic in tropical and recently in temperate climate areas, such as Corsica. The WHO estimates the number of people affected by schistosomiasis  >  240 million. Previously, we have shown that schistosomes influence the hepatic lipid metabolism. The present project aims to investigate the metabolic pathways by which S. mansoni eggs influence the hepatic lipid metabolism of the host.

    Methods Lipid metabolism was analyzed in liver tissue of S. mansoni-infected hamsters by western blot (WB), histological staining, mass spectrometry imaging (MSI), and immunohistochemistry (IHC). Non-infected hamsters and hamsters infected with clonal cercariae of one sex without egg production were used as controls. Additionally, human hepatoma cells (HepG2) were stimulated with soluble egg antigens (SEA) isolated from S. mansoni eggs.

    Results Although the total amount of neutral lipids in the liver of S. mansoni-infected hamsters decreases compared to non-infected hamsters, increased lipid accumulation occurs in the eggs and in perigranulomatous hepatocytes. In bisex-infected hamsters, the expression of key enzymes of lipid synthesis such as acetyl-CoA-carboxylase 1 (ACC1) and fatty acid synthase (FAS) was reduced in WB but increased in perigranulomatous hepatocytes. In contrast, enzymes involved in lipid catabolism, e.g. hepatic lipase (LIPC), were down-regulated globally but also in hepatocytes close to the granuloma. These findings have been verified in vitro.

    Conclusion Our data show a change in the composition of neutral lipids between S. mansoni-infected and control liver tissue. In addition, we observed increased lipid synthesis and accumulation as well as reduced lipid catabolism in perigranulomatous hepatocytes in the liver of infected hamsters. For the first time, we demonstrate that schistosomal eggs induce a modulation of the host lipid metabolism.


    #

    Publication History

    Article published online:
    04 January 2021

    © 2020. Thieme. All rights reserved.

    Georg Thieme Verlag KG
    Rüdigerstraße 14, 70469 Stuttgart, Germany