CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S227-S228
DOI: 10.1055/s-0041-1728463
Abstracts
Otology / Neurotology / Audiology

Immunological findings based on protein analysis of human perilymph samples

H Schmitt
1   Hannover Medical School, Department of Otolaryngology, Hannover
,
C Falk
3   Hannover Medical School, Institute of Transplant Immunology, Hannover
,
A Warnecke
1   Hannover Medical School, Department of Otolaryngology, Hannover
,
A Pich
4   Hannover Medical School, Core Facility Proteomics, Hannover
,
N Prenzler
1   Hannover Medical School, Department of Otolaryngology, Hannover
,
M Durisin
1   Hannover Medical School, Department of Otolaryngology, Hannover
,
T Lenarz
1   Hannover Medical School, Department of Otolaryngology, Hannover
› Author Affiliations
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    Background Besides genetic defects, also inflammatory processes are supposed to contribute to damaging processes in the inner ear leading to hearing loss (HL). Through progress in highly sensitive protein analytics by mass spectrometry (MS) and multiplex protein arrays (MPA), we could already analyse human perilymph (hPL) samples [1,2]. The aim of this study was to analyse hPL samples by both methods in parallel for defining the hPL proteome and the composition of cytokines, chemokines and growth factors of patients with different etiology of HL.

    Methods hPL samples of 98 CI-patients (partly included in [1,2]) have been analysed by MPA (human 48-cytokine plex). 62 patients suffering from Vestibular schwannoma, Meningitis, Enlarged vestibular aqueduct, otosclerosis, Ménière’s disease, CMV, Cx26 genetic defect, acute HL, or Waardenburg syndrome, for 36 patients the etiology was unknown. For 35 samples, additionally MS analysis was performed.

    Results In our published initial cohort with 44 hPL samples [1], we observed different inflammatory patterns with cytokines (IL-6,IL-1b,TNF-a) or endothelial proteins (VEGF,IGF-BP1). Here we could confirm these patterns that seem to be spread across different etiologies. By bioinformatics we could not clearly define any clustering of age, sex, etiology. The clustering of 8 single side deafness patients identified IGFBP-1, VEGF-A, endoglin, Ang-2, CXCL10, VCAM, TGF-a as potential discriminators compared to patients with bilateral HL.

    Conclusion Inflammatory processes and endothelial dysregulation may contribute to several forms of HL. The results of this protein based approach enables novel insights into the pathophysiology of HL that may allow a more targeted treatment.

    [1] Warnecke Front. Neurol. 2019

    [2] Schmitt J Proteome R. 2017

    Poster-PDF A-1149.pdf

    Cluster of Excellence of the German Research Foundation EXC 2177/1 “Hearing4all”


    #

    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    Dr. rer. nat. Schmitt Heike
    Cluster of Excellence of the German Research Foundation (DFG; “Deutsche Forschungsgemeinschaft “Hearing4all”
    Hannover

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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