CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S308
DOI: 10.1055/s-0041-1728931
Abstracts
Salivary Glands / Thyroid Gland: Salivary Glands

Neutrophil Extracellular Traps (NETs) and their role in the Development and Growth of Human Salivary Stones

M Schapher
1   Universitätsklinikum Erlangen, Hals-Nasen-Ohrenklinik, Kopf- und Halschirurgie, Erlangen
,
M Koch
1   Universitätsklinikum Erlangen, Hals-Nasen-Ohrenklinik, Kopf- und Halschirurgie, Erlangen
,
D Weidner
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
M Scholz
4   Universität Erlangen, Institut für Anatomie II, Erlangen
,
S Wirtz
5   Universitätsklinikum Erlangen, Medizinische Klinik 1, Gastroenterologie, Pneumologie und Endokrinologie, Erlangen
,
A Mahajan
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
I Herrmann
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
J Singh
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
J Knopf
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
M Leppkes
5   Universitätsklinikum Erlangen, Medizinische Klinik 1, Gastroenterologie, Pneumologie und Endokrinologie, Erlangen
,
C Schauer
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
A Grüneboom
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
C Alexiou
1   Universitätsklinikum Erlangen, Hals-Nasen-Ohrenklinik, Kopf- und Halschirurgie, Erlangen
,
G Schett
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
H Iro
1   Universitätsklinikum Erlangen, Hals-Nasen-Ohrenklinik, Kopf- und Halschirurgie, Erlangen
,
L Munoz
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
,
M Herrmann
2   Universitätsklinikum Erlangen, Medizinische Klinik 3, Rheumatologie, Erlangen
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    Content Salivary gland stones are the most common cause of obstructive salivary gland dieseases which are characterized by recurrent painful periprandial swelling of the affected gland. The obstructions often result in sialadenitis and the need for endoscopic or surgical intervention.

    Several theories exist which have tried to explain the mechanism behind the formation of sialoliths. In our studies, we can show by employing different techniques (immunohistochemistry, immunofluorescence, computed tomography scans and reconstructions, special dye techniques, bacterial genotyping and enzyme activity analyses) that neutrophil granulocytes externalize their chromatin, thereby establishing neutrophil extracellular traps (NETs) which initiate the formation and growth of sialoliths in humans. The neutrophil granulocyte extracellular DNA captures and aggregates debris and small crystals, followed by their mineralization. These mineralized surfaces subsequently trigger the formation of further NETs. A repeating process thereby creates alternating layers of dense mineral, predominantly calcium salt deposits, and DNA, resembling a ferroconcrete construction. The ongoing agglomeration and appositional growth of these structures promotes the development of macroscopic sialoliths that finally occlude the efferent ducts of the gland, causing clinical symptoms.

    These findings provide an entirely novel insight into the mechanism of sialolithogenesis: an immune system-mediated response essentially participates in the physicochemical process of concrement development.

    Poster-PDF A-1197.pdf

    Deutsche Forschungsgemeinschaft (DFG); European Union (EU); Interdisciplinary Center for Clinical Research (IZKF); Friedrich-Alexander Universität Erlangen-Nürnberg (FAU) Open Access Publishing; Optical Imaging Center Erlangen (OICE); Margarete Ammon Foundation Munich; Manfred Roth-Foundation Fürth; Volkswagen-Stiftung; ELAN of the FAU.


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    Conflict of interest

    Der Erstautor gibt keinen Interessenskonflikt an.

    Address for correspondence

    Dr. med. habil. Dipl.-Mol.Med. Univ. Schapher Mirco
    Universitätsklinikum Erlangen, Hals-Nasen-Ohrenklinik, Kopf- und Halschirurgie
    Erlangen

    Publication History

    Article published online:
    13 May 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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