CC BY-NC-ND 4.0 · Indian J Med Paediatr Oncol 2022; 43(S 01): S1-S19
DOI: 10.1055/s-0042-1755497
Abstracts

Noninvasive Imaging-Guided In Vivo Estimation of Photothermal Therapy Using Cancer-Targeted Gold-Solid-Lipid Nanomaterial

Chetna Patnaik
1   Molecular Functional Imaging Lab, ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai, India
2   NanoBios Lab, Department of Bioscience and Bioengineering, Indian Institute of Technology (IIT-B), Powai, Mumbai, India
,
Suditi Neekhra
3   Life Science, Homi Bhabha National Institute (HBNI), Mumbai, India
,
Shreya Dalvi
1   Molecular Functional Imaging Lab, ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai, India
,
Sabyasachi Chakrabarty
3   Life Science, Homi Bhabha National Institute (HBNI), Mumbai, India
,
B. Pradeep K. Reddy
3   Life Science, Homi Bhabha National Institute (HBNI), Mumbai, India
,
Rohit Srivastava
3   Life Science, Homi Bhabha National Institute (HBNI), Mumbai, India
,
Abhijit De
1   Molecular Functional Imaging Lab, ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai, India
2   NanoBios Lab, Department of Bioscience and Bioengineering, Indian Institute of Technology (IIT-B), Powai, Mumbai, India
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    *Correspondence to: ade@actrec.gov.in

    Background: Photothermal therapy (PTT) is a promising cancer treatment modality which employs near-infrared laser on gold-nanomaterials for localized heat-mediated tumor ablation. We estimate PTT effect of a novel CSC-targeted photothermal agent, that is, hyaluronic acid (HA)-tagged gold-solid-lipid nanoparticle (Au-SLN) on breast cancer (BC) cells and xenograft tumors.

    Methods: Au-SLN was surface functionalized with HA and characterized. In vitro uptake and biocompatibility of HA-Au-SLN over Au-SLN were done. To evaluate PTT efficacy in vivo, 4T1-FL2 BC orthotopic NOD-SCID mice were given PTT (750 nm NIR-laser, 650 mW, and 4 minutes) after intratumoral Au-SLN injection. Tumor regression was quantitated by 2D-BLI and DLIT of IVIS-spectrum.

    Results: HA-Au-SLN showed no shift in NIR absorbance efficiency. HA coating was confirmed by FTIR and TEM. In vitro studies showed significant cell death (>75%, p < 0.0001) within 2 minutes of PTT across BC cell lines. Enhanced uptake of HA-Au-SLN over Au-SLN was observed in MDA-MB-231 cell line. HA-Au-SLN showed higher biocompatibility over Au-SLN at concentrations beyond 100 µg/mL in L929 cell line. In concordance to planar imaging estimation, volumetric assessment by DLIT simulation signified a 104-fold dip in source signal upon photothermal insult, leading to complete regression in primary tumor.

    Conclusion: This is the first report of an actively targeted gold nanomaterial for PTT showing excellent antitumor efficacy bringing this therapy closer for clinical application.


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    No conflict of interest has been declared by the author(s).

    Publication History

    Article published online:
    22 August 2022

    © 2022. Indian Society of Medical and Paediatric Oncology. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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