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Planta Med 2003; 69(6): 561-563
DOI: 10.1055/s-2003-40645
Letter
© Georg Thieme Verlag Stuttgart · New York

Induction of Apoptosis and G2/M Cell Cycle Arrest by Gleditsioside E from Gleditsia sinensis in HL-60 Cells

Lei Zhong1 , Guiqing Qu1 , Ping Li1 , Jian Han1 , Dean Guo1
  • 1State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China
Further Information

Publication History

Received: October 28, 2002

Accepted: February 15, 2003

Publication Date:
16 July 2003 (online)

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Abstract

Gleditsioside E, a triterpene saponin isolated from Gleditsia sinensis, showed significant cytotoxicity against Bel-7402, BGC-823, HeLa, HL-60 and MCF-7 cell lines. The results of flow cytometry with annexin V-FITC/PI double staining proved that gleditsioside E mainly induced early apoptosis in HL-60 cells. Gleditsioside E treatment resulted in a prominent increase of the G2/M population in HL-60 cells, and an accumulation of the sub-G1 (hypoploid) peak was observed.

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Prof. Dr. Dean Guo

State Key Laboratory of Natural and Biomimetic Drugs

School of Pharmaceutical Sciences

Peking University

Xueyuan Road 38

Beijing 100083

P. R. China

Email: gda@bjmu.edu.cn

Fax: +86-10-62092700