RSS-Feed abonnieren
DOI: 10.1055/s-0030-1250583
© Georg Thieme Verlag KG Stuttgart · New York
NMR Fingerprinting for Analysis of Hoodia Species and Hoodia Dietary Products
Publikationsverlauf
received July 30, 2010
revised October 28, 2010
accepted Nov. 2, 2010
Publikationsdatum:
02. Dezember 2010 (online)
![](https://www.thieme-connect.de/media/plantamedica/201108/lookinside/thumbnails/10.1055-s-0030-1250583-1.jpg)
Abstract
Hoodia gordonii, a succulent plant growing in African arid regions, is used as a botanical dietary supplement for weight loss. The increasing concerns on the quality and safety of Hoodia products call for the needs of more science-based information, as well as objective and efficient tools for inspection. In the present study, NMR fingerprinting and multivariate analysis techniques were applied for the identification, discrimination, and quality analysis of Hoodia plant materials and commercial products. Four Hoodia species, namely H. gordonii (five authenticated samples), H. currorii (one authenticated sample), H. parviflora (three authenticated samples), and H. rushii (one authenticated sample), were investigated; the chemicals and characteristic spectral signals that made most contributions for their differentiations were revealed. With the aid of NMR fingerprint analysis, ten Hoodia products sold on the dietary supplement market were assessed for their chemical composition and quality. The study demonstrated that the NMR fingerprinting approach could be a promising and efficient tool for the authentication of botanicals.
Key words
NMR - fingerprinting - multivariate analysis - Hoodia species - Asclepiadaceae - botanical - dietary supplement
- Supporting Information for this article is available online at
- Supporting Information .
References
- 1 Mabberly D J. The plant-book. A portable dictionary of the vascular plants. Cambridge; Cambridge University Press 1997: 354
- 2 van Heerden F R, Marthinus H R, Maharaj V J, Vleggaar R, Senabe J V, Gunning P J. An appetite suppressant from Hoodia species. Phytochemistry. 2007; 68 2545-2553
- 3 Tibe O, Modise D M, Mogotsi K K. Potential for domestication and commercialization of Hoodia and Opuntia species in Botswana. Afr J Biotechnol. 2008; 7 1199-1203
- 4 Avula B, Wang Y H, Pawar R S, Shukla Y J, Smillie T J, Khan I A. A rapid method for chemical fingerprint analysis of Hoodia species, related genera, and dietary supplements using UPLC-UV-MS. J Pharm Biomed Anal. 2008; 48 722-731
- 5 Robb-Nicholson C. By the way, doctor. I've seen a lot of Internet ads for Hoodia, a natural supplement that suppresses your appetite. What do you know about it? Does it work, is it safe?. Harv Womens Health Watch. 2008; 15 8
- 6 Rumalla C S, Avula B, Shukla Y J, Wang Y H, Pawar R S, Smillie T J, Khan I A. Chemical fingerprint of Hoodia species, dietary supplements, and related genera by using HPTLC. J Sep Sci. 2008; 31 3959-3964
- 7 Avula B, Wang Y H, Pawar R S, Shukla Y J, Khan I A. Chemical fingerprinting of Hoodia species and related genera: chemical analysis of oxypregnane glycosides using high-performance liquid chromatography with UV detection in Hoodia gordonii. J AOAC Int. 2007; 90 1526-1531
- 8 Teng R, Junankar P R, Bubb W A, Rae C, Mercier P, Kirk K. Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by 1H NMR spectroscopy. NMR Biomed. 2009; 22 292-302
- 9 Powers R. NMR metabolomics and drug discovery. Magn Reson Chem. 2009; 47 S2-S11
- 10 Verpoorte R, Choi Y, Kim H. NMR-based metabolomics at work in phytochemistry. Phytochem Rev. 2007; 6 3-14
- 11 van der Kooy F, Maltese F, Choi Y H, Kim H K, Verpoorte R. Quality control of herbal material and phytopharmaceuticals with MS and NMR based metabolic fingerprinting. Planta Med. 2009; 75 763-775
- 12 Ward J L, Baker J M, Beale M H. Recent applications of NMR spectroscopy in plant metabolomics. FEBS J. 2007; 274 1126-1131
- 13 Pawar R S, Shukla Y J, Khan I A. New calogenin glycosides from Hoodia gordonii. Steroids. 2007; 72 881-891
- 14 Pawar R S, Shukla Y J, Khan S I, Avula B, Khan I A. New oxypregnane glycosides from appetite suppressant herbal supplement Hoodia gordonii. Steroids. 2007; 72 524-534
- 15 Son H S, Hwang G S, Kim K M, Kim E Y, van den Berg F, Park W M, Lee C H, Hong Y S. 1H NMR-based metabolomic approach for understanding the fermentation behaviors of wine yeast strains. Anal Chem. 2009; 81 1137-1145
- 16 Wiklund S, Johansson E, Sjostrom L, Mellerowicz E J, Edlund U, Shockcor J P, Gottfries J, Moritz T, Trygg J. Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models. Anal Chem. 2008; 80 115-122
- 17 van Heerden F R. Hoodia gordonii: a natural appetite suppressant. J Ethnopharmacol. 2008; 119 434-437
- 18 Shukla Y J, Pawar R S, Ding Y, Li X C, Ferreira D, Khan I A. Pregnane glycosides from Hoodia gordonii. Phytochemistry. 2009; 70 675-683
- 19 Lindon J C, Nicholson J K. Analytical technologies for metabonomics and metabolomics, and multi-omic information recovery. Trends Analyt Chem. 2008; 27 194-204
- 20 Kang J, Choi M Y, Kang S, Kwon H N, Wen H, Lee C H, Park M, Wiklund S, Kim H J, Kwon S W, Park S. Application of a 1H nuclear magnetic resonance (NMR) metabolomics approach combined with orthogonal projections to latent structure-discriminant analysis as an efficient tool for discriminating between Korean and Chinese herbal medicines. J Agric Food Chem. 2008; 56 11589-11595
Prof. Dr. Ikhlas A. Khan
National Center for Natural Products Research
Research Institute of Pharmaceutical Sciences
University of Mississippi
P. O. Box 1848
University, MS 38677
USA
Telefon: +1 66 29 15 78 21
Fax: +1 66 29 15 79 89
eMail: ikhan@olemiss.edu
Dr. Jianping Zhao
National Center for Natural Products Research
Thad Cochran Research Center
University of Mississippi
P. O. Box 1848
University, MS 38677
USA
Telefon: +1 66 29 15 71 51
Fax: +1 66 29 15 79 89
eMail: jianping@olemiss.edu
- www.thieme-connect.de/ejournals/toc/plantamedica