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DOI: 10.1055/s-0032-1327901
In Vivo Protective Activity of Styrax camporum Hydroalcoholic Extract against Genotoxicity Induced by Doxorubicin and Methyl Methanesulfonate in the Micronucleus and Comet Assays
Publikationsverlauf
received 13. Juni 2012
revised 10. September 2012
accepted 08. Oktober 2012
Publikationsdatum:
19. Dezember 2012 (online)
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Abstract
Styrax camporum Pohl is a tall shrub or a tree with small white flowers, which grows in the states of São Paulo and Minas Gerais and is popularly used for the treatment of gastroduodenal diseases. Considering this last fact, the aim of this study was to evaluate the genotoxic potential of S. camporum hydroalcoholic extract and its influence on genotoxicity induced by doxorubicin and methyl methanesulfonate in Swiss mice using the micronucleus and comet assays, respectively. The animals were treated by gavage with different doses of the extract (250, 500, and 1000 mg/kg body weight). For antigenotoxicity assessment, different doses of the S. camporum extract were administered simultaneously with doxorubicin (micronucleus test; 15 mg/kg) and methanesulfonate (comet assay; 40 mg/kg). The results showed that the S. camporum extract itself was not genotoxic in the mouse micronucleus or comet assay. The number of micronucleated polychromatic erythrocytes was significantly lower in animals treated with the S. camporum extract and doxorubicin when compared to animals treated only with doxorubicin. In the comet assay, the S. camporum extract, at the doses tested, significantly reduced the extent of DNA damage in liver cells induced by methanesulfonate. The putative activity of the active compounds of S. camporum extract may explain the effect of this plant on genotoxicity induced by doxorubicin and methanesulfonate.
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References
- 1 da Costa Lopes L, Albano F, Laranja GAT, Alves LM, Silva LFM, Souza GP, Araujo IM, Nogueira-Neto F, Felzenszwalb I, Kovary K. Toxicological evaluation by in vitro and in vivo assays of an aqueous extract prepared from Echinodorus macrophyllus leaves. Toxicol Lett 2000; 116: 189-198
- 2 Fritsch PW, Morton CM, Chen T, Meldrum C. Phylogeny and biogeography of the Styracaceae. Int J Plant Sci 2001; 162: 95-116
- 3 Li QL, Li BG, Qi HY, Gao XP, Zhang GL. Four new benzofurans from seeds of Styrax perkinsiae . Planta Med 2005; 71: 847-851
- 4 Moraes AC, Bertanha CS, Gimenez VM, Groppo M, Silva ML, Cunha WR, Januário AH, Pauletti PM. Development and validation of a high-performance liquid chromatography method for quantification of egonol and homoegonol in Styrax species. Biomed Chromatogr 2011; Epub ahead of print DOI: 10.1002/bmc.1744.
- 5 Lorenzi H. Árvore Brasileiras. Manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 2nd. edition. Nova Odessa: Plantarum; 2002
- 6 Bacchi EM, Sertié JAA. Antiulcer action of Styrax camporum and Caesalpinia ferrea in rats. Planta Med 1994; 60: 118-120
- 7 Bacchi EM, Sertié JAA, Villa N, Katz H. Antiulcer action and toxicity of Styrax camporum and Caesalpinea ferrea . Planta Med 1995; 61: 204-207
- 8 De Flora S, Ferguson LR. Overview of mechanisms of cancer chemopreventive agents. Mutat Res 2005; 591: 8-15
- 9 Zhou S, Koh HL, Gao Y, Gong ZY, Lee EJD. Herbal bioactivation: the good, the bad and the ugly. Life Sci 2004; 74: 935-968
- 10 Fernandes DC, Regasini LO, Vellosa JC, Pauletti PM, Castro-Gamboa I, Bolzani VS, Oliveira OM, Silva DH. Myeloperoxidase inhibitory and radical scavenging activities of flavones from Pterogyne nitens . Chem Pharm Bull 2008; 56: 723-726
- 11 Venkatesh P, Shantala B, Jagetia GC, Rao K, Baliga MS. Modulation of doxorubicin-induced genotoxicity by Aegle marmelos in mouse bone marrow: a micronucleus study. Integr Cancer Ther 2007; 6: 42-53
- 12 MacGregor JT, Wehr CM, Gould DH. Clastogen-induced micronuclei in peripheral blood erythrocytes: the basis of an improved micronucleus test. Environ Mutagen 1980; 2: 509-514
- 13 Mersch-Sundermann V, Kassie F, Bohmer S, Lu WQ, Wohlfahrth R, Sobel R, Brunn HE, ElSohly MA, Ross SA, Stahl T. Extract of Toxicodendron quercifolium caused genotoxicity and antigenotoxicity in bone marrow cells of CD1 mice. Food Chem Toxicol 2004; 42: 1611-1617
- 14 Waters MD, Brady AL, Stack HF, Brockman HE. Antimutagenicity profiles for some model compounds. Mutat Res 1990; 238: 57-85
- 15 Franke SIR, Prá D, Silva J, Erdtmann B, Henriques JAP. Possible repair action of vitamin C on DNA damage induced by methyl methanesulfonate, cyclophosphamide, FeSO4 in mouse blood cells in vivo . Mutat Res 2005; 583: 75-84
- 16 Burlinson B, Tice RR, Speit G, Agurell E, Brendler-Schwaab SY, Collins AR, Escobar P, Honma M, Kumaravel TS, Nakajima M, Sasaki YF, Thybaud V, Uno Y, Vasquez M, Hartmann A. Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroup. In Vivo Comet Assay Workgroup, part of the Fourth International Workgroup on Genotoxicity Testing. Mutat Res 2007; 627: 31-35
- 17 Collins AR, Oscoz AA, Brunborg G, Gaivão I, Giovannelli L, Kruszewski M, Smith CC, Stetina R. The comet assay: topical issues. Mutagenesis 2008; 23: 143-151
- 18 Tsuboy MS, Angeli JP, Mantovani MS, Knasmüller S, Umbuzeiro GA, Ribeiro L. Genotoxic, mutagenic and citotoxic effects of the commercial dye CI Disperse Blue 291 in the human hepatic cell line Hep G2. Toxicol In Vitro 2007; 8: 1650-1655
- 19 Injac R, Strukelj B. Recent advances in protection against doxorubicin-induced toxicity. Technol Cancer Res Treat 2008; 7: 497-516
- 20 Beranek DT. Distribution of methyl and ethyl adducts following alkylation with monofunctional alkylating agents. Mutat Res 1990; 231: 11-30
- 21 Wyatt MD, Pittman DL. Methylating agents and DNA repair responses: methylated bases and sources of strand breaks. Chem Res Toxicol 2006; 19: 1580-1594
- 22 Zhang F, Bartels MJ, Pottenger LH, Bhaskar B, Gollapudi BB. Differential adduction of proteins vs. deoxynucleosides by methyl methanesulfo-nate and 1-methyl-1-nitrosourea in vitro. Rapid Commun Mass Spectrom 2005; 19: 438-448
- 23 OʼConnor TR, Boiteux S, Laval J. Ring-opened 7-methylguanine residues in DNA are a block to in vitro DNA synthesis. Nucleic Acids Res 1988; 16: 5879-5894
- 24 Ma W, Resnick MA, Gordenin DA. Apn1 and Apn2 endonucleases prevent accumulation of repair-associated DNA breaks in budding yeast as revealed by direct chromosomal analysis. Nucleic Acids Res 2008; 36: 1836-1846
- 25 Baute J, Depicker A. Base excision repair and its role in maintaining genome stability. Crit Rev Biochem Mol Biol 2008; 43: 239-276
- 26 Liu H, Lightfoot R, Stevens JL. Activation of heat shock factor by alkylating agents is triggered by glutathione depletion and oxidation of protein thiols. J Biol Chem 1996; 271: 4805-4812
- 27 Pauletti PM, Araújo AR, Young MCM, Giesbrecht AM, Bolzani VS. Nor-lignans from the leaves of Styrax ferrugineus (Styracaceae) with antibacterial and antifungical activity. Phytochemistry 2000; 55: 597-601
- 28 Pauletti PM, Araújo AR, Bolzani VS. Triterpenos de Styrax camporum (Styracaceae). Quim Nova 2002; 25: 349-352
- 29 Teles HL, Hemerly JP, Pauletti PM, Pandolfi JRC, Araújo AR, Valentini SR, Young MC, Bolzani VS, Silva DH. Cytotoxic lignans from the stems of Styrax camporum (Styracaceae). Nat Prod Res 2005; 19: 319-323
- 30 Min BS, Oh SR, Ahn KS, Kim JH, Lee J, Kim DY, Kim EH, Lee HK. Anti-complement activity of norlignans and terpenes from the stem bark of Styrax japonica . Planta Med 2004; 70: 1210-1215
- 31 Takanashi M, Takizawa Y. New benzofurans related to egonol from immature seeds of Styrax obassia . Phytochemistry 1988; 27: 1224-1226
- 32 Takanashi M, Takizawa Y. Isolation of new egonol compounds from seeds of Styrax japonica Sieb. et Zucc. J Oleo Sci 2002; 51: 151-155
- 33 Naczk M, Shahidi F. Extraction and analysis of phenolic in food. J Chromatogr 2004; 1054: 95-111
- 34 Shrubsole MJ, Jin F, Daí Q, Shu X, Potter JD, Hebert JR. Dietary folate intake and breast cancer risk: results from the Shanghai Breast Cancer Study. Cancer Res 2001; 61: 7136-7141
- 35 Rajeshkumar NV, Joy KL, Kuttan G, Ramsewak RS, Nair MG, Kuttan R. Antitumour and anticarcinogenic activity of Phyllanthus amarus extract. J Ethnopharmacol 2002; 81: 17-22
- 36 Chun SS, Vatem DA, Lin YT, Shetty K. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori . Process Biochem 2005; 40: 809-816
- 37 Abalea V, Cillard J, Dubos MP, Sergent O, Cillard P, Morel I. Repair of iron-induced DNA oxidation by the flavonoid myricetin in primary rat hepatocyte cultures. Free Radic Biol Med 1999; 26: 1457-1466
- 38 Lehraiki A, Attoumbré J, Bienaimé C, Matifat F, Bensaddek L, Nava-Saucedo E, Fliniaux MA, Ouadid-Ahidouch H, Baltora-Rosset S. Extraction of lignans from flax seed and evaluation of their biological effects on breast cancer MCF-7 and MDA-MB231 cell lines. J Med Food 2010; 13: 834-841
- 39 Resende FA, Tomazella IM, Barbosa LC, Ponce M, Furtado RA, Pereira AC, Bastos JK, Andrade E, Silva ML, Tavares DC. Effect of the dibenzilbuttyrolactone lignan (−)-hinokinin on doxorubicin and methyl methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts. Mutat Res 2010; 700: 62-66
- 40 Medola JF, Cintra VP, Silva EPP, Royo VA, da Silva R, Saraiva J, Albuquerque S, Bastos JK, Andrade E, Silva ML, Tavares DC. (−)-Hinokinin causes antigenotoxicity but not genotoxicity in peripheral blood of Wistar rats. Food Chem Toxicol 2007; 45: 638-642
- 41 Quiles JL, Huertas JR, Battino M, Mataix J, Ramírez-Tortosa MC. Antioxidant nutrients and adriamycin toxicity. Toxicology 2002; 180: 79-95
- 42 Knasmüller S, Steinkellner HBJ, Majer EC, Nobis G, Scharf G, Kassie F. Search for dietary antimutagens and anticarcinogens: methodological aspect and extrapolation aspect and extrapolation problems. Food Chem Toxicol 2002; 40: 1051-1062