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Thromb Haemost 1992; 67(03): 331-334
DOI: 10.1055/s-0038-1648442
Original Articles
Schattauer GmbH Stuttgart

Cell Shape Change and Cytosolic Ca2+ in Human Umbilical-Vein Endothelial Cells Stimulated with Thrombin

Haruhiko Sago
The Clinical Dysmorphology Laboratory, National Children’s Medical Research Center, Tokyo, Japan
,
Kazuso linuma
The Clinical Dysmorphology Laboratory, National Children’s Medical Research Center, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 01 May 1991

Accepted after revision 12 September 1991

Publication Date:
03 July 2018 (online)

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Summary

We quantified thrombin-induced endothelial cells shape change and investigated the role of Ca2+ in such shape change. We used the fluorescent Ca2+ indicator, fura2, to measure both shape change as cell size and intracellular free Ca2+ ([Ca2+]i), in cultured human umbilical-vein endothelial cells (HUVEC). Thrombin induced concentration-dependent decreases in cell size (percentage of cell size at 6 min after stimulation with 0.01 U/ml, 0.1 U/ml, or 1 U/ml thrombin) was 90.1 ± 1.5%, 78.1 ± 2.4%, and 40.9 ± 2.4%, respectively. Thrombin also increased [Ca2+]i in a concentration-dependent manner. Both depletion of extracellular Ca2+, and also the addition of W5, a calmodulin antagonist, inhibited thrombin-induced size reduction. These results indicate an association between shape change and [Ca2+]i mobilization in human endothelial cells stimulated by thrombin.

 

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