Subscribe to RSS
DOI: 10.1055/s-0038-1651216
Metabolic Studies of Vitamin K1-14C and Menadione-14C in the Normal and Hepatectomized Rats[*]
Publication History
Publication Date:
27 June 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/196801/lookinside/thumbnails/10-1055-s-0038-1651216-1.jpg)
Summary
The metabolism of vitamin K1- 14C and menadione-14C (vitamin K3-14C) was studied in normal and hepateetomized rats. After the administration of menadione, about 70% of the 14C was excreted in the urine in 24 hrs in both types of rats. Two urinary metabolites were identified by enzymatic hydrolysis: one a glucuronide and the other a sulfate of reduced menadione. Thus, the liver is not necessary for the metabolism of menadione. In the vitamin K1 studies, the intact rats excreted only 10% of the 14C and the hepatectomized rats excreted less than 0.5%. The retention of vitamin K1 may explain its superiority over menadione as an antidote for overdosages of oral anticoagulants.
* This investigation was supported in part by Research Grants AM-3932 and HE-9863 from the National Institutes of Health, Public Health Service, and by a grant from the Medical Research Council of Canada.
-
References
- 1 Bollman J.L, Van Hook E. A simplified two-stage hepatectomy in the rat, J. appl. Physiol.(In press.).
- 2 Boyd E.J, Warner E.D. Effect of vitamin K on dicumarol-induced hypoprothrombinemia in rats. J. Lab. clin. Med. 1948; 33: 1431
- 3 Ghandrasekhar N, Hickie R.A, Millar G.J. The effect of dicumarol on prothrombin time in rats and its relation to vitamin K content of liver. Canad. J. Physiol. Pharmacol. 1965; 43: 639
- 4 Cromer jr. H.E, Barker N.W. The effect of large doses of menadione bisulfite (synthetic vitamin K) on excessive hypoprothrombinemia induced by dicumarol. Proc. Mayo Clin. 1944; 19: 217
- 5 Grindlay J.H, Mann F.G. Removal of the liver of the dog: An experimental surgical technique. Surgery 1952; 31: 900
- 6 Jürgens R. Wirkung von Vitamin K1 auf die Dicumarol-Hypoprothrombinämie und die Acceleratorfaktoren am lebergeschädigten Kaninchen und nach Leberexstirpation an der Katze. Acta haemat. (Basel) 1952; 7: 143
- 7 Jürgens R, Studer A. Beeinflussung der Bildung des Prothrombins im Tierversuch. Schweiz. med. Wschr. 1952; 82: 1119
- 8 Lee G.G, Trevoy L.W, Spinks J.W.T, Jaques L.B. Dicumarol labelled with C14 . Proc. Soc. exp. Biol. (N. Y.) 1950; 74: 151
- 9 Losito R, Owen jr. G.A, Eunice V. Flock Metabolism of [14C] menadione. Biochemistry 1967; 6: 62
- 10 Losito R, Owen jr. G.A, Eunice V. Flock Vitamin K1 studies in the isolated perfused rat liver. (Abstr.) Fed. Proc. 1967; 26: 774
- 11 Meade R.C, Stiglitz R.A. Improved solvent systems for liquid scintillation counting of body fluids and tissues. Int. J. appl. Radiat. 1962; 13: 11
- 12 Millar G.J, Henreit M, Jaques L.B. Antagonistic and antidotal action of vitamins K1 and K3 versus dicumarol in dogs. Proc. Soc. exp. Biol. (N. Y.) 1965; 119: 1093
- 13 Overman R.S, Stahmann M.A, Link K.P. Studies on the hemorrhagic sweet clover disease. VIII. The effect of 2-methyl-l, 4-naphthoquinone and 1-ascorbic acid upon the action of 3,3’-methylenebis (4-hydroxycoumarin) on the prothrombin time of rabbits. J. biol. Chem. 1942; 145: 155