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Thromb Haemost 1997; 77(02): 394-398
DOI: 10.1055/s-0038-1655974
Original Article
Schattauer GmbH Stuttgart

C5a Induces Tissue Factor Activity on Endothelial Cells

Kei Ikeda
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Kohei Nagasawa
2   The Department of Internal Medicine, Saga Medical School, Saga, Japan
,
Takahiko Horiuchi
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Tomomi Tsuru
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Hiroaki Nishizaka
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
,
Yoshiyuki Niho
1   The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
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Publikationsverlauf

Received 30. April 1996

Accepted after revision 24. Oktober 1996

Publikationsdatum:
10. Juli 2018 (online)

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Summary

Tissue factor (TF) is an integral membrane glycoprotein that serves as a cofactor for blood coagulation factor VIIa. The induction of TF on the surface of endothelial cells is initiated by various stimuli including lipopolysaccharide, interleukin-1β, and tumor necrosis factor α. We have demonstrated that recombinant human C5a induces TF activity in a dose-dependent fashion in human umbilical vein endothelial cells (HUVEC). Peak activity (4.9-fold increase) was obtained 3-6 h after treatment with 10 μM C5a. TF mRNA as assessed by RT-PCR method was also significantly increased (3.75-fold) after 3 h incubation with C5a, suggesting that C5a induces TF activity on HUVEC, at least in part, by enhancing the level of TF mRNA. The increase in TF activity by C5a was inhibited by methylprednisolone. The induction of TF on endothelial cells by C5a may represent one of many potential interrelationships between the inflammatory and coagulation schemes.

 

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