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Thromb Haemost 1982; 47(01): 036-040
DOI: 10.1055/s-0038-1657120
Original Article
Schattauer GmbH Stuttgart

Comparative Study of the Activity of High and Low Molecular Weight Urokinase in the Presence of Fibrin

M Samama
1   The Laboratoire Central d'Hématologie, Hôtel-Dieu, Paris, France
,
M Castel
1   The Laboratoire Central d'Hématologie, Hôtel-Dieu, Paris, France
,
O Matsuo
2   The Department of Physiology, Miyazaki Medical College, Miyazaki, Japan
,
M Hoylaerts
3   The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Leuven, Belgium
,
H R Lijnen
3   The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Leuven, Belgium
› Institutsangaben
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Publikationsverlauf

Received 19. August 1981

Accepted 16. Dezember 1981

Publikationsdatum:
13. Juli 2018 (online)

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Summary

The fibrinolytic or thrombolytic activity of low molecular weight urokinase (LMW-UK) and high molecular weight urokinase (HMW-UK) is not significantly different when measured in a bovine fibrin plate method, in a circulating plasma system containing a 125I-labelled human fibrin clot, or on 125I-fibrin films in culture plates using normal or α2-antiplasmin depleted human plasma.

In a human fibrin plate method however HMW-UK was found to be more active than LMW-UK. In a purified system on human 125I-fibrin films the activation of native or modified human plasminogen by HMW-UK was also found to be more effective than by LMW-UK.

Using a clot lysis test system we did not observe a different inhibition of LMW-UK and HMW-UK upon incubation in human plasma. This is in contrast with previous reports that HMW-UK is inhibited more rapidly in human plasma than LMW-UK.

In a purified system the inhibition rate of LMW-UK and HMW-UK by α2-antiplasmin is the same (rate constants at 25ΰC of 167 ± 9 M−1s−1 and 171 ± 5 M−1s−1 respectively).

The clinical trials available at present used doses of urokinase which were in excess of those required to obtain a maximal fibrinolytic effect. This might explain why in these trials no difference was observed between the thrombolytic effect of LMW-UK and HMW-UK, while in vitro HMW-UK appeared to be more effective. However, one should always be careful to extrapolate in vitro observations as such to the in vivo situation encountered during thrombolytic therapy.

Key words

High and low molecular weight urokinase - Comparison of the fibrinolytic activity - Inhibition in human plasma - Inhibition by α2-antiplasmin
 

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