PDF herunterladen
Thromb Haemost 1986; 56(03): 288-292
DOI: 10.1055/s-0038-1661668
Original Article
Schattauer GmbH Stuttgart

Comparative Study of Size, Total Protein, Fibrinogen and 5-HT Content of Human and Canine Platelet Density Subpopulations

Diego Mezzano
The Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile
,
Eduardo Aranda
The Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile
,
Arnaldo Foradori
The Department of Hematology-Oncology, School of Medicine, Catholic University, Santiago, Chile
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 18. März 1986

Accepted after revision 26. August 1986

Publikationsdatum:
18. Juli 2018 (online)

Auch verfügbar auf
  als PDF herunterladen  Lizenzen und Reprints

Summary

The size, total protein, fibrinogen and 5-HT content were evaluated in density subpopulations of human and canine platelets fractionated in linear arabinogalactan gradients. The methodology was assessed to ascertain that platelet separation was by density and to discard artifactual changes and platelet release during the procedure. EDTA or PGEi increased the size of human PRP-platelets, but not of dog platelets. In humans, high density (HD) platelets were 1.26 times larger and contained 1.88 times more fibrinogen, 2.23 times more 5-HT and 1.37 times more protein than low density (LD) platelets; in dogs, these density cohorts did not differ in protein content, but LD platelets were 1.29 times larger and had 1.33 times more fibrinogen and 5-HT than HD platelets. These findings suggest that cell density is mostly dependent on the protein content per unit volume of platelets (and not on dense bodies). The differences in fibrinogen and 5-HT content between HD and LD cohorts in humans and dogs may be related to platelet age. The difference in volume between HD and LD platelets in dogs is of uncertain interpretation.

Keywords

Platelet density - Size - Fibrinogen - 5-HT - Age
 

  • References

  • 1 Karpatkin S. Heterogeneity of rabbit platelets. VI. Further resolution of changes in platelet density, volume, and radioactivity following cohort labelling with 75Selenomethionine. Brit J Haematol 1978; 39: 459-469
    CrossrefPubMedSuche in Google Scholar
  • 2 Corash L, Costa JL, Shafer B, Donlon JA, Murphy D. Heterogeneity of human whole blood platelet subpopulations. III. Density-dependent differences in subcellular constituents. Blood 1984; 64: 185-193
    CrossrefPubMedSuche in Google Scholar
  • 3 Packham MA, Guccione MA, O’Brien KM. Duration of the effect of aspirin on the synthesis of thromboxane by density subpopulations of rabbit platelets stimulated with thrombin. Blood 1985; 66: 287-290
    PubMedSuche in Google Scholar
  • 4 Boneu B, Vigoni F, Boneu A, Caranobe C, Sié P. Further studies on the relationship between platelet buoyant density and platelet age. Am J Hematol 1982; 13: 239-246
    CrossrefPubMedSuche in Google Scholar
  • 5 Mezzano D, Hwang KL, Catalano P, Aster RH. Evidence that platelet buoyant density, but not size, correlates with platelet age in man. Am J Hematol 1981; 11: 61-76
    CrossrefPubMedSuche in Google Scholar
  • 6 Martin JF, Penington DG. The relationship between the age and density of circulating 51Cr-labelled platelets in the sub-human primate. Thromb Res 1983; 30: 157-164
    CrossrefPubMedSuche in Google Scholar
  • 7 McDonald JW D, Ali M. Recovery of cyclooxygenase activity after aspirin in populations of platelets separated on stractan density gradients. Prostaglandins Leukotrienes and Medicine 1983; 12: 245-252
    CrossrefPubMedSuche in Google Scholar
  • 8 Corash L. Platelet density heterogeneity. Blood 1985; 65: 779-781 (Letter)
    PubMedSuche in Google Scholar
  • 9 Mezzano D, Aranda E, Foradori A, Rodriguez S, Lira P. Kinetics of platelet density subpopulations in splenectomized mongrel dogs. Am J Hematol 1984; 17: 373-382
    CrossrefPubMedSuche in Google Scholar
  • 10 Mezzano D, Aranda E, Rodríguez S, Foradori A, Lira P. Increase in density and accumulation of serotonin by human aging platelets. Am J Hematol 1984; 17: 11-21
    CrossrefPubMedSuche in Google Scholar
  • 11 Mezzano D, Hwang K, Aster RH. Characteristics of total platelet populations and of platelets isolated in platelet-rich plasma. Transfusion 1982; 22: 197-202
    CrossrefPubMedSuche in Google Scholar
  • 12 Corash L, Tan H, Gralnick HR. Heterogeneity of human whole blood platelet subpopulations. I. Relationship between buoyant density, cell volume and ultrastructure. Blood 1977; 49: 71-87
    PubMedSuche in Google Scholar
  • 13 Beaufay H, Jacques P, Baudhuin P, Sellinger OZ, Berthet J, de Duve C. Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients. Biochem J 1964; 92: 184-205
    CrossrefPubMedSuche in Google Scholar
  • 14 von Behrens WE. Mediterranean macrothrombocytopenia. Blood 1975; 46: 199-208
    PubMedSuche in Google Scholar
  • 15 Mundschenk DD, Connelly DP, White JG, Brunning RD. An improved technique for the electronic measurement of platelet size and shape. J Lab Clin Med 1976; 88: 301-315
    PubMedSuche in Google Scholar
  • 16 Markwell MA K, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87: 206-210
    CrossrefPubMedSuche in Google Scholar
  • 17 Drummond AH, Gordon JL. Rapid, sensitive microassay for platelet 5-HT. Thrombos Diathes Haemorrh 1974; 31: 366-367
    PubMedSuche in Google Scholar
  • 18 Mezzano D, Aranda E, Urzúa J, Lema G, Habash J, Irarrézabal MJ, Pereira J. Changes in platelet beta-thromboglobulin, fibrinogen, albumin, 5-hydroxytrypt-amine ATP and ADP during and after surgery with extracorporeal circulation in man. Am J Hematol 1986; 22: 133-142
    CrossrefPubMedSuche in Google Scholar
  • 19 Leven RM, Schick PK, Budzynsky AZ. Fibrinogen biosynthesis in isolated guinea pig megakaryocytes. Blood 1985; 65: 501-504
    PubMedSuche in Google Scholar
  • 20 Kaplan KL, Owen J. Radioimmunoassays of platelet alpha-granule proteins. In Measurements of Platelet Function Harker LA, Zimmerman TS. (Eds) Chapter 6 115-125 Churchill Livingstone; Edinburgh: 1983
  • 21 Van Oost BA, Timmermans AP M, Sixma JJ. Evidence that platelet density depends on the alpha-granule content in platelets. Blood 1984; 63: 482-485
    PubMedSuche in Google Scholar
  • 22 Vicic WJ, Weiss HJ. Evidence that platelet alpha-granules are a major determinant of platelet density: studies in storage pool deficiency. Thromb Haemostas 1983; 50: 878-880
    PubMedSuche in Google Scholar
  • 23 Van Oost BA, van Hien-Hagg IH, Veldhuyzen BF E, Timmermans AP M, Sixma JJ. Determination of the density distribution of human platelets. Methodological aspects and comparison with other tests for platelet activation. Thromb Haemostas 1982; 47: 239-243
    PubMedSuche in Google Scholar
  • 24 Martin JF, Shaw T, Heggie J, Penington DG. Measurement of the density of human platelets and its relationship to volume. Br J Haematol 1983; 54: 337-352
    CrossrefPubMedSuche in Google Scholar
  • 25 Packham MA, Perry DW, Kinlough-Rathbone RL, Rand ML, Guccione MA, Evans RM, Mustard JF. Effects on the buoyant density of rabbit platelets of ADP and agents that increase the concentration of cyclic AMP. Blood 1985; 65: 564-570
    PubMedSuche in Google Scholar
  • 26 Boneu B, Sié P, Caranobe C, Nouvel C, Biermé R. Malondial-dehyde (MDA) re-appearance in human platelet density subpopulations after a single intake of aspirin. Thromb Res 1980; 19: 609-620
    CrossrefPubMedSuche in Google Scholar
  • 27 Costa JL, Smith MA, Jaffe RM. Exchange of serotonin (5-HT) in dog and human platelet dense bodies. Fed Proc 1976; 35: 806
    PubMedSuche in Google Scholar
  • 28 Chao FC, Campo SR, Kenney DM. Localization of fibrin converted by thrombin from released platelet fibrinogen. Blood 1980; 55: 187-194
    PubMedSuche in Google Scholar
  • 29 Gerrard JM, Phillips DR, Rao GH R, Plow EF, Walz DA, Ross R, Harker LA, White JG. Biochemical studies of two patients with the gray platelet syndrome. Selective deficiency of platelet alpha granules. J Clin Invest 1980; 66: 102-109
    CrossrefPubMedSuche in Google Scholar
  • 30 Kaplan KL, Broekman MJ, Chernoff A, Lesznik GR, Drillings M. Platelet alpha-granule proteins: studies on release and subcellular localization. Blood 1979; 53: 604-618
    PubMedSuche in Google Scholar
  • 31 Sander HJ, Slot JW, Bouma BN, Bolhuis PA, Pepper DS, Sixma JJ. Immunocytochemical localization of fibrinogen, platelet factor 4, and beta thromboglobulin in thin frozen sections of human blood platelets. J Clin Invest 1983; 72: 1277-1287
    CrossrefPubMedSuche in Google Scholar
  • 32 Wencel-Drake JD, Painter RG, Zimmerman TS, Ginsberg MH. Ultrastruc-tural localization of human platelet thrombospondin, fibrinogen, fibronectin and von Willebrand factor in frozen thin section. Blood 1985; 65: 929-938
    PubMedSuche in Google Scholar
  • 33 Belloc F, Hourdillé P, Fialon P, Boisseau MR, Soria J. Fibrinogen synthesis by megakaryocyte rich human marrow cell concentrates. Thromb Res 1985; 38: 341-351
    CrossrefPubMedSuche in Google Scholar
  • 34 Karpatkin M, Howard L, Karpatkin S. Studies of the origin of platelet-associated fibrinogen. J Lab Clin Med 1984; 104: 223-237
    PubMedSuche in Google Scholar
  • 35 Santoso S, Zimmermann U, Neppert J, Mueller-Eckhardt C. Receptor patching and capping of platelet membranes induced by monoclonal antibodies. Blood 1986; 67: 343-349
    PubMedSuche in Google Scholar
  • 36 Rendu F, Nurden AT, Lebret M, Caen JP. Relationship between mepacrine-labelled dense body number, platelet capacity to accumulate 14C-5-HT and platelet density in the Bernard-Soulier and Hermansky-Pudlak syndromes. Thromb Haemostas 1979; 42: 694-704
    PubMedSuche in Google Scholar
  • 37 Costa JL, Reese TS, Murphy DL. Serotonin storage in platelets: estimation of storage-packet size. Science 1974; 183: 537-538
    CrossrefPubMedSuche in Google Scholar
  • 38 Thompson CB, Love DG, Quinn PG, Valeri CR. Platelet size does not correlate with platelet age. Blood 1983; 62: 487-494
    CrossrefPubMedSuche in Google Scholar