Planta Med 2019; 85(18): 1449-1450
DOI: 10.1055/s-0039-3399798
Main Congress Poster
Poster Session 1
© Georg Thieme Verlag KG Stuttgart · New York

Production of phenolic acids in shoot cultures of black aronia (Aronia melanocarpa) cultivated in RITA bioreactors

P Kubica
1   Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College,, 9 Medyczna Str., 30-688 Kraków, Poland
,
A Pałka
1   Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College,, 9 Medyczna Str., 30-688 Kraków, Poland
,
A Szopa
1   Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College,, 9 Medyczna Str., 30-688 Kraków, Poland
,
H Ekiert
1   Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College,, 9 Medyczna Str., 30-688 Kraków, Poland
› Author Affiliations
Further Information

Publication History

Publication Date:
20 December 2019 (online)

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Background: Black aronia (Aronia melanocarpa) is plant species of North-American origin cultivated in Poland. Fruits of this plant contain some subgroups of antioxidants – phenolic acids and anthocyanins important in phytotherapy and also cosmetology [1].

Our previous studies with different types of in vitro cultures of black aronia (agar and agitated cultures) documented the high biosynthetic potential of the cells for production of phenolic acids [2],[3].

Aim: The aim of the present study was the establishment of shoot cultures in temporary immersion system – in RITA bioreactors and analysis of phenolic acids in cultured in vitro biomass and media. The cultures were maintained on Murashige and Skoog medium [4] enriched in 1 mg/l BAP and 1 mg/l NAA, for 4 and 8 weeks (3 series). In methanolic extracts of collected biomasses and culture media samples the LC-DAD analysis of 26 phenolic acids was performed [5].

Results: In biomasses extracts the presence of 11 phenolic acids was confirmed. The quantitatively dominant compounds were: isochlorogenic acid, cryptochlorogenic acid, 3,4-dihydroxyphenylacetic acid and 3-phenylacetic acid (max. 236.16; 153.96; 151.80 and 101.04 mg/100gDW, respectively). Extracts from the culture media were found to contain no metabolites. Higher total content of phenolic acids was confirmed offer 4-week growth cycles (786.88 mg/100gDW and 380.66 mg/100gDW, respectively).

Conclusion: The obtained results documented for the first time the satisfactory production of some phenolic acids in shoot cultures of black aronia cultivated in RITA bioreactors. The obtained total content of phenolic acids are interesting from practical point of view.

 

  • References

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