Immunomodulation in Cholesteatoma

Introduction The etiopathogenesis of chronic epitympanic otitis media and it’s proliferative destructive course with possible complications such as destruction of bony structures with hearing loss, vestibular dysfunction, facial nerve paralysis and intracranial complications are inconclusive. At present time surgery is the first-line therapy. Current studies are increasingly looking at the innate immune system.

Methods Our studies were carried out on HMECC cells, from a TNF knockout mouse model. Cholestatoma and healthy ear canal skin was removed during ear surgery. The expression analysis were carried out at the gene and protein level, using Affymetrix Microarray and qPCR with TNF as the major target for therapy evaluation. By means of TUNEL staining, immunohistochemistry on cryocuts and applying in Situ Apoptosis Detection Kit, the level of apoptosis through TNF was evaluated.

Results The uncontrolled proliferative cholestatoma growth shows an immunomodulatory profile with high regulation of various gene networks, especially TNF downstream and upstream signaling pathways and their molecules. TNF is modeled both inflammatory and apoptotically and therefore is suitable as a possible therapeutic approach in various models.

Conclusions Cholestatoma is regulated by immunomodulatory pathways.

DFG-Förderung LE 2363/2-1 (AL)

Poster-PDF A-1940.PDF

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Artikel online veröffentlicht:
10. Juni 2020