Cryopreservation of Papaver somniferum Cell Suspension Cultures*

Laurie J. Friesen; Kutty K. Kartha; Nick L. Leung; Pat Englund; Kenneth L. Giles; Jong Park; David D. Songstad

doi:10.1055/s-2006-960017

Planta Med 1991; 57(1): 53-55
DOI: 10.1055/s-2006-960017

Papers

Cryopreservation of Papaver somniferum Cell Suspension Cultures*

Laurie J. Friesen¹ , Kutty K. Kartha¹ , Nick L. Leung¹ , Pat Englund² , Kenneth L. Giles² , Jong Park² , David D. Songstad²

¹National Research Council Canada, Plant Biotechnolgy Institute, Saskatoon, Saskatchewan, S7N OW9, Canada
²Vipont Research Laboratories, c/o National Research Council, Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan, S7N OW9, Canada

* NRCC No. 32447

Abstract

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Abstract

The effect of culture age and various cooling rates on the survival and regrowth of cryopreserved cell cultures of Papaver somniferum L. is described. Survival and regrowth following cryopreservation, in liquid nitrogen, occurred only with 4 and 5 day old cultures. No regrowth was observed in 3 or 6 day old cultures. Cooling rate had a significant effect on survival following cryopreservation with an optimum cooling rate of 0.5°C min^-1.

Key words

Papaver somniferum - cryopreservation - cell suspension cultures - sanguinarine production - high-yielding cell lines

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