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DOI: 10.1055/s-2007-967124
Anthelmintic Activity of Aporphine Alkaloids from Cissampelos capensis
Publication History
Received: November 30, 2006
Accepted: January 20, 2007
Publication Date:
16 February 2007 (online)
Abstract
Two known aporphine alkaloids, (S)-dicentrine (1) and (S)-neolitsine (2), have been isolated from the MeOH extract of the aerial parts of Cissampelos capensis (Menispermaceae). The structures of these compounds were elucidated by NMR and MS analysis and comparison to literature data. These compounds were isolated by bioassay-guided fractionation using the Haemonchus contortus larval development assay. Compounds 1 and 2 exhibited EC90 values (concentration at which 90 % loss of larval motility is observed) of 6.3 and 6.4 μg/mL, respectively. In an in vivo assay, administration of 1 resulted in 67 % reduction of worm counts in mice at 25 mg/kg when dosed orally.
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Dr. Sheo B. Singh
Natural Products Chemistry
Merck Research Laboratories
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Email: sheo_singh@merck.com