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Drug Res (Stuttg) 2023; 73(03): 137-145
DOI: 10.1055/a-1974-9132
Original Article

Cysteamine Attenuate Intestinal Reperfusion Injury Induced by Occlusion of Mesenteric Artery by Enhancing Intracellular Thiol Activities

Babatunde Alabi
1   Department of Pharmacology & Therapeutics, Faculty of Basic Clinical Sciences, Bowen University, Osun State, Nigeria
,
Olugbenga Iwalewa
2   Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria
,
Temidayo Omobowale
3   Department of Veterinary Medicine, University of Ibadan, Nigeria
,
Adeolu Adedapo
4   Department of Veterinary Pharmacology & Toxicology, University of Ibadan, Nigeria
,
Opeyemi Hammed
5   Department of Physiology, Faculty of Basic Medical Science, Ladoke Akintola University of Technology, Nigeria
,
Richard Ajike
5   Department of Physiology, Faculty of Basic Medical Science, Ladoke Akintola University of Technology, Nigeria
,
Oladele Afolabi
5   Department of Physiology, Faculty of Basic Medical Science, Ladoke Akintola University of Technology, Nigeria
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Abstract

Background Ischemia/reperfusion has been reported to further damage the intestine reperfusion injury (IRI) and cause multiple distal organ dysfunction through oxidative stress, inflammation, and apoptosis. Cysteamine is known to inhibit oxidative stress, inflammatory cytokines and apoptosis. This experiment was designed to evaluate the role of cysteamine against IRI in rats

Methods Thirty-two Wistar rat strains were assigned to four groups: sham, Intestinal-reperfusion injury (IRI), 50 mg/kg and 100 mg/kg cysteamine treatment IRI. A 5 cm segment of terminal ileum was twisted 360° clockwise along the mesentery for 45 minutes to induce ischemia before detorsion. Tissues were preserved for biochemical evaluation and histology 4 hours after detorsion. Activities of GPx, GSH, protein and non-protein thiol, H2O2, MDA were evaluated. Serum concentration of nitrite, MPO, ALT, AST TNF-alpha and IL-6 were measured. Caspase 3 and bax were evaluated by immunohistochemistry. Statistical significance was set as p<0.05

Results Significant (p<0.05) increase in H2O2, MDA and nitrite but reduction in GPx, GSH, protein thiol and non-protein thiol in the IRI rats was reversed by 50 and 100 mg/kg cysteamine. Serum MPO, TNF-α, IL6, AST and ALT was significantly elevated in IRI while the rats treated with cysteamine showed a significant decrease (p<0.05) in the activities of these inflammatory and hepatic injury markers.

Conclusion Cysteamine mitigate IRI by enhancing intracellular antioxidant defense system, inhibiting inflammatory mediators and intestinal tissue expression of pro-apoptotic protein.

Key words

Detorsion - cytokines - apoptosis - hepatic injury - terminal ileum - mesenteric artery - cysteamine antioxidants


Publication History

Received: 17 September 2022

Accepted: 28 October 2022

Article published online:
27 December 2022

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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