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Planta Med 2023; 89(07): 709-717
DOI: 10.1055/a-1997-5566
Biological and Pharmacological Activity
Original Papers

Curative Effect and Mechanisms of Radix Arnebiae Oil on Burn Wound Healing in Rats

Ting Gao
1   Department of Pharmaceutical Preparation, General Hospital of Ningxia Medical University, Yinchuan, China
2   School of Basic Medicine, Ningxia Medical University, Yinchuan, China
,
Yu Zhao
3   College of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Yuna Zhao
1   Department of Pharmaceutical Preparation, General Hospital of Ningxia Medical University, Yinchuan, China
,
Yanping He
1   Department of Pharmaceutical Preparation, General Hospital of Ningxia Medical University, Yinchuan, China
,
Qi Huang
4   Department of Biochip Research Center, General Hospital of Ningxia Medical University, Yinchuan, China
,
Jianhong Yang
3   College of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Liming Zhang
3   College of Pharmacy, Ningxia Medical University, Yinchuan, China
,
Jing Chen
1   Department of Pharmaceutical Preparation, General Hospital of Ningxia Medical University, Yinchuan, China
› Author AffiliationsSupported by: Key Research and Development Projects of Ningxia No. 2022BEG03141
Supported by: Natural Science Foundation of Ningxia Province No. 2022AAC03579
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Abstract

Radix arnebiae oil (RAO) is a clinically useful traditional Chinese medical formula with outstanding curative effects on burns. However, the mechanism of the effect of RAO on wound healing remains unclear. The present study investigates the molecular mechanisms of the potential curative effect of RAO on wound healing. The concentrations of the main constituents, shikonin, imperatorin, and ferulic acid in RAO detected by HPLC were 24.57, 3.15, and 0.13 mg/mL, respectively. A rat burn model was established, and macroscopic and histopathological studies were performed. RAO significantly accelerated wound closure and repair scarring, increased superoxide dismutase activities, and reduced malondialdehyde. RAO also downregulated interleukin (IL)-6, IL-1β and tumor necrosis factor-α in wound tissues and increased secretion of vascular endothelial growth factor, epidermal growth factor, and transforming growth factor (TGF)-β1. RAO increased the gene expression of TGF-β1, type I and III collagen, and increased the protein expression of TGF-β1 and phosphorylation of PI3K and Akt. In conclusion, RAO likely promotes wound healing via antioxidant and anti-inflammatory activities and increases re-epithelization. Activation of the TGF-β1/PI3K/Akt pathway may play an important role in the healing efficacy of RAO. These findings suggest that RAO could be a promising alternative local treatment for burn wound healing.

Key words

Radix arnebiae oil - wound healing - TGF-β1/PI3K/Akt pathway


Publication History

Received: 19 July 2022

Accepted after revision: 09 December 2022

Accepted Manuscript online:
13 December 2022

Article published online:
17 April 2023

© 2023. Thieme. All rights reserved.

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