A New Cytotoxic Trichothecene Macrolide from the Endophyte Myrothecium roridum

Abstract

A new cytotoxic roridin-type trichothecene macrolide named roritoxin E (1) was characterized from the solid culture of Myrothecium roridum IFB-E091 (residing originally inside Artemisia annua root), together with the known compounds lumichrome (2), (22E,24S)-cerevisterol (3), and (22E,24R)-6β-methoxy-ergosta-7,22-diene-3β,5α-diol (4). The structure of the new macrolide (1) was elucidated by a combination of spectroscopic (UV, IR, MS, and 1D and 2D NMR) analyses. Compound 1 was demonstrated to be inhibitory in vitro against the gastric carcinoma SGC-7901 and hepatocarcinoma SMMC-7721 cell lines, with IC₅₀ values of 0.26 and 10.54 µg/mL, respectively. 5-Fluorouracil co-assayed as a positive control had an IC₅₀ value of 6.66 µg/mL against SGC-7901 cells, and it demonstrated only a 9.98 % growth inhibition against SMMC-7721 cells at 10 µg/mL.

Key words

Myrothecium roridum - Tuberculariaceae - endophyte - trichothecene macrolide - roritoxin E

References

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