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Planta Med 2003; 69(5): 452-456
DOI: 10.1055/s-2003-39699
Original Paper
Analytical Methods
© Georg Thieme Verlag Stuttgart · New York

Quantification of the Flavonoid Glycosides in Passiflora incarnata by Capillary Electrophoresis

Elke Marchart1 , Liselotte Krenn1 , Brigitte Kopp1
  • 1Institute of Pharmacognosy, University of Vienna, Vienna, Austria
Further Information

Publication History

Received: September 3, 2002

Accepted: December 7, 2002

Publication Date:
12 June 2003 (online)

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Abstract

Capillary electrophoresis has been applied for the separation and quantification of the flavonoids in Passiflorae herba. Separations were performed using 25 mM sodium borate with 20 % methanol (pH 9.5). For the quantification quercetin 3-O-arabinoside was used as internal standard. The method was applied to the determination of the flavonoid glycosides in 10 different commercial samples of the drug and showed similar flavonoid patterns, but differences concerning the single and total amounts of flavonoids. The total flavonoid contents determined with the new method correlated satisfactorily with those achieved by the spectrophotometric assay according to the European Pharmacopoeia.

Key words

Capillary electrophoresis - flavonoids - C-glycosylflavones - Passiflora incarnata L. - Passifloraceae - passionflower

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Prof. Dr. B. Kopp

Institute of Pharmacognosy

PharmaCenterVienna

University of Vienna

Althanstr. 14

1090 Vienna

Austria

Email: brigitte.kopp@univie.ac.at

Fax: +43-1-4277-55256

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