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Horm Metab Res 1972; 4(2): 72-77
DOI: 10.1055/s-0028-1094103
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© Georg Thieme Verlag KG Stuttgart · New York

The In Vitro 14C-Cyclic AMP Production by Normal Human Adipose Tissue in Response to Some Hormones and in Uncontrolled and Controlled Diabetic Adipose Tissue

A. H. Kissebah [*] , T. R. Fraser
  • Endocrine Unit, Department of Medicine, Royal Postgraduate Medical School, London, England
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Publication History

Publication Date:
07 January 2009 (online)

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Abstract

The 14C-c-AMP production by human subcutaneous adipose tissue biopsies has been measured in response to noradrenaline and in groups of 12 uncontrolled and 9 insulin-controlled diabetics and contrasted with that of 10 non-diabetic controls obtained during an operation.

With in vitro incubated normal human adipose tissue, measurements following the addition of noradrenaline showed a tenfold increase in the cellular content of 14C-c-AMP at 10-40 min after the noradrenaline, and preceding their release of glycerol. Addition of insulin to the incubate prevented both these effects.

Subcutaneous adipose tissue from uncontrolled diabetics showed increased adenyl cyclase activity and decreased phosphodiesterase activity, while tissue from insulin-controlled diabetics showed these enzyme activities in the normal range.

Key words

C-AMP - Human Adipose Tissue - Diabetes - Noradrenaline - Adenyl Cyclase - Phosphodiesterase

1 Present address: Alexander Simpson Laboratory for Metabolic Research, St. Mary's Hospital Medical School, London W.2, England