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Planta Med 1972; 21(4): 347-352
DOI: 10.1055/s-0028-1099563
© Georg Thieme Verlag Stuttgart · New York

COMPARATIVE STUDY BETWEEN DIFFERENT METHODS USED FOR ESTIMATION OF PYRETHRINS AND A NEW SUGGESTED METHOD

S. I. Balbaa, E. M. Abdel–Kader, S. M. Abdel–Wahab, A. Y. Zaki, A. M. El–Shamy
  • Pharmacognosy Department, Faculty of Pharmacy, Cairo University, U. A. R.
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Publication Date:
14 January 2009 (online)

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Abstract

A comparative study between the methods commonly used in the determination of the pyrethrin content in pyrethrum flowers viz., Seil, Mercury reduction and the sulphur colorimetric methods was untertaken.

A new Chromatographie method for the estimation of pyrethrin I and pyrethrin II is described and the results are compared with the forementioned methods.

The light petroleum extract of the flowers was chromatographed on Kieselgel G plates: the spots of „pyrethrin I” and „pyrethrin II” were eluted. A colour reagent consisting of phosphoric acid : acetic anhydride (80 : 20 V/V) was heated with the eluate for 2 or 3 minutes at 100° C. (This gives a pink colour only with pyrethrin I and pyrethrin II) and this colour was measured in S. P. 500 spectrophotometer at 550 mμ.

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