Increased Acylphosphatase Levels in Erythrocytes, Muscle and Liver of Tri-Iodothyronine Treated Rabbits

Summary

To explore a possible role of acylphosphatase in the regulation of energy metabolism, we measured this enzyme's activity and content in skeletal muscle, liver and erythrocytes of normal and tri-iodothyronine treated rabbits. Besides acylphosphatase we assayed (Na⁺ + K⁺)-ATPase, Ca²⁺-ATPase and several enzymes of carbohydrate metabolism. Acylphosphatase activity in erythrocytes rose steadily during treatment with tri-iodothyronine (25 μg/Kg per day for 5 weeks), and its increase occurred earlier and was much more pronounced than that of other soluble enzymes. In erythrocytes of treated animals (Na⁺ + K⁺)-ATPase declined whereas Ca²⁺-ATPase activity increased, in agreement with previously reported findings. In muscle and liver of the treated animals acylphosphatase activity was about twice as high as in the controls; in these tissues we found also increased activities for (Na⁺ + K⁺)-ATPase, fructose-1,6-bisphosphatase and glucose-6-phosphatase. In any case, among the enzymes we examined, acylphosphatase was one of the most strongly and regularly stimulated by the treatment. Furthermore we observed, through an immunochemical procedure, that there was a congruence between increases in acylphosphatase activity and content. On the basis of these results we conclude that the rise in acylphosphatase levels in treated animals is probably due to its increased biosynthesis. The possible significance of these findings in the metabolic modifications associated with hyperthyroidism are discussed.

Key words

Acylphosphatase - Tri-Iodothyronine - Energy Metabolism