Gluconeogenesis from Nucleosides in Perfused Rat Livers

Summary

1. In fed rats various nucleosides and their monophosphates caused a strong glucose release from perfused livers.

2. This effect was further studied with inosine and found depending on the rat strain used; it was much less pronounced in the Wistar strain WU than in the Hannover Wistar or the Sprague Dawley strain.

In animals fasted 48 hours less glucose was released in the presence of inosine; no difference was observed between the various strains.

3. In fasted rats of all three strains inosine led to gluconeogenesis probably by its ribose moiety. In addition, 60 % of the hepatic glucose output caused by inosine was derived from glycogen in fed Hannover Wistar rats.

4. The reduction of the hepatic blood flow after the addition of nucleosides, which could cause glycogenolysis by hypoxia, cannot explain their glucose releasing effect.

Key-Words:

Gluconeogenesis - Nucleosides - Purine Bases - Urate Synthesis