Homeopathy 2011; 100(04): 275-287
DOI: 10.1016/j.homp.2011.05.008
Research Review
Copyright © The Faculty of Homeopathy 2011

Use of homeopathic preparations in experimental studies with abiotically stressed plants

Tim Jäger
1   Institute of Complementary Medicine KIKOM, University of Bern, Switzerland
2   Research Institute of Organic Agriculture, Frick, Switzerland
,
Claudia Scherr
3   Society for Cancer Research, Hiscia Institute, Arlesheim, Switzerland
,
Devika Shah
3   Society for Cancer Research, Hiscia Institute, Arlesheim, Switzerland
,
Vera Majewsky
1   Institute of Complementary Medicine KIKOM, University of Bern, Switzerland
4   Clinic for Animal Reproduction, Freie Universität Berlin, Germany
,
Lucietta Betti
5   Department of Agro-Environmental Science and Technology, University of Bologna, Italy
,
Grazia Trebbi
5   Department of Agro-Environmental Science and Technology, University of Bologna, Italy
,
Leoni Bonamin
6   Laboratory of Cell and Molecular Biology, University Paulista, São Paulo, Brazil
,
Ana Paula Simões-Wüst
7   Paracelsus Hospital Richterswil, Switzerland
,
Ursula Wolf
1   Institute of Complementary Medicine KIKOM, University of Bern, Switzerland
,
Meinhard Simon
8   Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Germany
,
Peter Heusser
9   Center for Integrative Medicine, University of Witten/Herdecke, Germany
,
Stephan Baumgartner
1   Institute of Complementary Medicine KIKOM, University of Bern, Switzerland
3   Society for Cancer Research, Hiscia Institute, Arlesheim, Switzerland
9   Center for Integrative Medicine, University of Witten/Herdecke, Germany
› Author Affiliations

Subject Editor:
Further Information

Publication History

Received04 January 2011
revised20 April 2011

accepted11 May 2011

Publication Date:
20 December 2017 (online)

Background: Experimental research on the effects of homeopathic treatments on impaired plants was last reviewed in 1990.

Objectives: To compile a systematic review of the existing literature on basic research in homeopathy with abiotically stressed plants using predefined criteria.

Methods: The literature search was carried out on publications that reported experiments on homeopathy using abiotically stressed whole plants, seeds, plant parts and cells from 1920 to 2010. Outcomes had to be measured by established procedures and statistically evaluated. Using of a Manuscript Information Score (MIS) we identified those publications that provided sufficient information for proper interpretation (MIS ≥ 5). A further evaluation was based on the use of adequate controls to investigate specific effects of homeopathic preparations and on the use of systematic negative control experiments.

Results: A total of 34 publications with abiotically stressed plants was identified, published between 1965 and 2010. The 34 publications described a total of 37 experimental studies. Twenty-two studies included statistics, 13 had a MIS ≥ 5, 8 were identified with adequate controls and 4 with negative control experiments. Significant and reproducible effects with decimal and centesimal potencies were found, including dilution levels beyond Avogadro’s number. One experimental model was independently assessed by another research team and yielded inverted results compared to the original trial.

Conclusions: Abiotically stressed plant models seem to be a useful approach to investigate homeopathic basic research questions, but more experimentation and especially more independent replication trials are needed. Systematic negative control experiments should be implemented on a routine basis to exclude false-positive results.

 
  • References

  • 1 Majewsky V., Arlt S., Shah D. et al. Use of homeopathic preparations in experimental studies with healthy plants. Homeopathy 2009; 98 (04) 228-243.
  • 2 Betti L., Trebbi G., Majewsky V. et al. Use of homeopathic preparations in phytopathological models and in field trials: a critical review. Homeopathy 2009; 98 (04) 244-266.
  • 3 Scofield M. Homeopathy – and its potential role in agriculture – a critical review. Biol Agric Hortic 1984; 2: 1-50.
  • 4 Majerus M. Kritische Begutachtung der wissenschaftlichen Beweisführung in der homöopathischen Grundlagenforschung. Thesis, Tierärztliche Hochschule Hannover, Germany, 1990.
  • 5 Albrecht H., van Wijk R., Dittloff F. A new database on basic research in homeopathy. Homeopathy 2002; 91: 162-165.
  • 6 Baumgartner S., Heusser P., Thurneysen A. Methodological standards and problems in preclinical homeopathic potency research. Forsch Komplementärmed 1998; 5 (01) 27-32.
  • 7 Auquière J.P., Moens P. Recherches de l’action de dilutions homéopathiques sur les végéteaux: I. Action du CuSO4 5CH sur blé intoxiqué au CuSO4 1 ‰. J Pharm Belg 1981; 36 (05) 303-320.
  • 8 Auquière JP, Moens P, Linster T, Speileux M. Recherche de l’action de dilutions homéopathiques sur les végétaux. Unpublished report, Louvain-la-Neuve, 1988.
  • 9 Auquière J.P., Moens P., Martin P.L. Recherche de l’action de dilutions homéopathiques sur les végéteaux: II. Action du CuSO4 14DH sur la Moutarde blanche (Sinapis alba L. var. dialba) intoxiquée au CuSO4 0.1 et 0.2 ‰. J Pharm Belg 1982; 37 (02) 117-134.
  • 10 Betti L., Brizzi M., Nani D., Peruzzi M. Effect of high dilutions of Arsenicum album on wheat seedlings from seed poisoned with the same substance. Br Hom J 1997; 86 (April): 86-89.
  • 11 Binder M., Baumgartner S., Thurneysen A. The effects of a 45x potency of Arsenicum album on wheat seedling growth – a reproduction trial. Forsch Komplementarmed Klass Naturheilkd 2005; 12 (05) 284-291.
  • 12 Boiron J. Action d’une dilution hahnemannienne arsenicale sur la croissance du blé intoxiqué à l’arsenic. Ann Homéopath Fr 1965; 7 (04) 253-258.
  • 13 Boiron J. Action de dilutions 5CH-7CH et 15CH de Sulfate de Cuivre sur la respiration de Salvinia natans. In: L.M.H.I.: 28th Congress of the International Homeopathic Medical League, Wien, 1973, pp 597–602.
  • 14 Boiron J., Abecassis J., Reynaud J. Etude de l’action d’une 15CH de sulfate de cuivre sur la germination de grains de blé subintoxiqués par cette substance. Ann Homéopath Fr 1978; 20 (04) 293-302.
  • 15 Boiron J., Marin A. Action des doses infinitésimales (15CH) d’une substance sur la cinétique d’élimination de cette même substance au cours de la culture de graines préalablemtent intoxiquées. Ann Homéopath Fr 1967; 9 (02) 121-130.
  • 16 Boiron J., Marin J. Action d’une 15CH de CuSO4 sur la cinétique d’élimination de cette substance au cours de la culture “d’embryons” préalablement intoxiqués. Ann Homéopath Fr 1968; 10 (03) 219-226.
  • 17 Boiron J., Marin M. Action d’une 15CH de sulfate de cuivre sur la culture de Chlorella vulgaris . Ann Homéopath Fr 1975; 17 (04) 367-372.
  • 18 Boiron J., Reynaud J. Démonstration de l’activité de dilutions 5-7-9 et 15CH de Cuprum sulfuricum sur la Respiration de Salvinia natans L. Ann Homéopath Fr 1975; 17: 499-507.
  • 19 Brizzi M., Biondi C., Lazzarato L., Betti L. Analisi esplorativa dell’effetto di soluzioni ultramolecolari di triossido di Arsenico sullo sviluppo vegetativo in vitro di plantule di grano. Statistica 2002; 62 (03) 515-522.
  • 20 Brizzi M., Elia V., Trebbi G., Nani D., Peruzzi M., Betti L. The efficacy of ultramolecular aqueous dilutions on a wheat germination model as a function of heat and aging-time. eCAM 2009
  • 21 Brizzi M., Lazzarato L., Nani D., Borghini F., Peruzzi M., Betti L. A biostatistical insight into the As2O3 high dilution effects on the rate and variability of wheat seedling growth. Forsch Komplementarmed Klass Naturheilkd 2005; 12 (05) 277-283.
  • 22 Brizzi M., Nani D., Peruzzi M., Betti L. Statistical analysis of the effect of high dilutions of arsenic in large dataset from a wheat germination model. Br Hom J 2000; 89: 63-67.
  • 23 Carvalho L.M., Casali V.W.D., Cecon P.R., Lisboa S.P., Souza M.A. Effect of homeopathy on recovery of feverfew plants [Tanacetum parthenium (L.) Schultz Bip] under water stress. Rev Bras Plantas Med 2004; 6: 20-27.
  • 24 Flemming H. Potenzieren – ein Weg, die Gegenwirkung der Stoffe zu erschliessen?. Element Naturwiss 1974; 20 (01) 26-32.
  • 25 Graviou E. Action d’une 15 ème centésimale de CuSO4 sur la respiration de pois intoxiqués au cuivre. Ann Homéopath Fr 1968; 10 (03) 227-228.
  • 26 Graviou E. Moment, sens et intensité de l’action d’une haute dilution sur un materiél végétal préintoxiqué. Ann Homéopath Fr 1980; 22 (02) 145-162.
  • 27 Graviou E. Dosage de l’action d’une 15CH de sulfate de cuivre Comparaison des résultats dans le temps. Ann Homéopath Fr 1981; 23 (03) 5-23.
  • 28 Graviou E. Problème de l’optimum de succussions en fonction du moment de la préparation de dilutions cuivriques. Homéopath Fr 1982; 24 (01) 29-34.
  • 29 Graviou E., Biron M.A. Action d’une 15CH de sulfate de cuivre sur la culture de Chlorella vulgaris . Ann Homéopath Fr 1971; 13 (07) 539-548.
  • 30 Jäger T., Scherr C., Simon M., Heusser P., Baumgartner S. Effects of homeopathic Arsenicum album, nosode, and gibberellic acid preparations on the growth rate of arsenic-impaired duckweed (Lemna gibba L.). ScientificWorldJournal 2010; 10: 2112-2129.
  • 31 Kovac H., Muhry F., Novic S., Moser M. Das Wachstum von Weizenkeimlingen nach Zugabe von toxischen Substanzen. Mitt Inst Strukt Med Forsch 1991; 3: 43-63.
  • 32 Lahnstein L., Binder M., Thurneysen A. et al. Isopathic treatment effects of Arsenicum album 45x on wheat seedling growth – further reproduction trials. Homeopathy 2009; 98 (04) 198-207.
  • 33 Moss V., Roberts J., Simpson H. The effect of copper sulphate on the growth of the alga Chlorella . Br Hom J 1977; LXVI (03) 169-177.
  • 34 Netien G. Action de doses infinitésimales de sulfate de cuivre sur des plantes préalablement intoxiquées par cette substance. Ann Homéopath Fr 1966; 4: 130-136.
  • 35 Netien G., Graviou E. Action d’une 15CH de sulfate de cuivre sur du materiel végétal préintoxiqué. Importance de la rythmologie pour cette étude. Ann Homéopath Fr 1979; 21 (04) 415-427.
  • 36 Netien G., Graviou E., Marin A. Action de doses infinitésimales de sulfate de cuivre sur des plantes préalablement intoxiquées par cette substance. Ann Homéopath Fr 1965; 7 (04) 248-252.
  • 37 Noiret R., Claude M. Etude enzymatique du grain de froment intoxiqué par CuSO4 et traité par différentes dilutions hahnémanniennes de la même substance. Rev Belg d’Hom 1976; IX (01) 461-496.
  • 38 Noiret R., Glaude M. Expérimentation des acides nucléiques ARN et ADN sur les graines de froment (inhibition de croissance et stimulation). Rev Belg d’Hom 1979; 11 (02) 71-82.
  • 39 Projetti M.L., Guillemain J., Tetau M. Effets curatifs et préventifs de dilutions homéopathiques de sulfate de cuivre appliquées à des racines de lentilles pré-ou post-intoxiquées. Cah Biothér 1985; 22 (88) 21-27.
  • 40 Tighe M. Homeopathy on cress – a pre-clinical method development using a simple plant growth model. Thesis, University of Westminster, London, UK, 2005.
  • 41 Jäger T., Scherr C., Simon M., Heusser P., Baumgartner S. Development of a test system for homeopathic preparations using impaired duckweed (Lemna gibba L.). J Altern Complement Med 2011; 17 (04) 315-323.
  • 42 Betti L., Trebbi G., Zurla M., Nani D., Peruzzi M., Brizzi M. A review of three simple plant models and corresponding statistical tools for basic research in homeopathy. ScientificWorldJournal 2010; 10: 2330-2347.
  • 43 Begon M., Townsend C., Harper J. Ecology: from individuals to ecosystems. Malden, USA: Wiley-Blackwell; 2005.