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Int J Sports Med 2013; 34(03): 268-273
DOI: 10.1055/s-0032-1312581
Immunology
© Georg Thieme Verlag KG Stuttgart · New York

Exercise Intensity and Lymphocyte Subset Apoptosis

J. W. Navalta
1   Kinesiology and Nutrition Sciences, University of Nevada, Las Vegas, Las Vegas, United States
,
S. Lyons
2   Kinesiology, Recreation, and Sport, Western Kentucky University, Bowling Green, United States
,
J. Prestes
3   Graduation program on Physical Education, Catholic University of Brasilia, Brasilia, Brazil
,
S. W. Arnett
2   Kinesiology, Recreation, and Sport, Western Kentucky University, Bowling Green, United States
,
M. Schafer
2   Kinesiology, Recreation, and Sport, Western Kentucky University, Bowling Green, United States
,
G. L. Sobrero
2   Kinesiology, Recreation, and Sport, Western Kentucky University, Bowling Green, United States
› Author Affiliations
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Publication History



accepted after revision 02 April 2012

Publication Date:
05 October 2012 (online)

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Abstract

This investigation assessed the lymphocyte subset response to increasing intensity. Participants completed an exertion test (VO2max), and later performed a 10-min run at 76% VO2max, 5-min at 87%, and run to exhaustion at 100% intensity. Blood was sampled at rest, following each intensity, and 1-h post. Cell concentration, apoptosis (annexin V) and migration (CX3CR1) were evaluated in CD4+, CD8+, and CD19+ subsets. Relative data were analyzed using 1-way ANOVA with significance at P≤0.05. Absolute changes from rest (Δ baseline) were calculated for exercise conditions. CX3CR1 displayed relative changes 1-h post, (CD8+ Pre=58%, Post=68%, 1 h-Post=37%, P=0.04) (CD19+ Pre=1.9%, Post=3.2%, 1 h-Post=5.2%, P=0.02). No relative changes were noted for subsets and annexin V. Absolute changes revealed that CD4+/annexin V+ and CD8+/annexin V+ significantly increased at 76%,(P<0.01). Significant absolute increases were observed in CD4+/CX3CR1 at 87% VO2max, and at 87% and 100% VO2max in CD8+/CX3CR1 (P<0.01). Subsets respond differently with intensity with respect to cell count, and markers of apoptosis and cell migration. CD4+ and CD8+ appear to be prone to apoptosis with moderate exercise, but significant increases in migration at higher intensities suggests movement of these cells from the vasculature in postexercise measurements.

Key words

leukocyte subfractions - lymphocytosis - programmed cell death - fractalkine - muscular activity - annexin V
 

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