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Semin Reprod Med 2013; 31(01): 049-055
DOI: 10.1055/s-0032-1331797
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Embryonic Stem Cells from Blastomeres Maintaining Embryo Viability

Irina Klimanskaya
1   Advanced Cell Technology, Inc., Marlborough, Massachusetts
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Publication History

Publication Date:
17 January 2013 (online)

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Abstract

A wide variety of cell and tissue types that are sought in regenerative medicine can be generated from embryonic stem cells (ESCs), and currently two derivatives of human embryonic stem cells (hESCs) have entered human clinical trials. However, the ethical controversy surrounding this technology, which uses preimplantation human embryos to generate cell lines, is limiting research and the development of new therapies. Several new technologies such as induced pluripotent cells or parthenogenetically derived pluripotent cells hold great promise, but more research is needed before their derivatives can be proven to be safe and functional for use in human patients. The blastomere biopsy-based technique allows the derivation of human ESClines without sacrificing a human embryo and was shown to be robust and produce safe and functional derivatives of therapeutic value.

Keywords

pluripotent cells - human embryonic stem cells - iPS - ES - hES - differentiated derivatives - parthenogenesis - reprogramming
 

  • References

  • 1 Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981; 292 (5819) 154-156
    CrossrefPubMedSearch in Google Scholar
  • 2 Thomson JA, Itskovitz-Eldor J, Shapiro SS , et al. Embryonic stem cell lines derived from human blastocysts. Science 1998; 282 (5391) 1145-1147
    CrossrefPubMedSearch in Google Scholar
  • 3 Carpenter MK, Rosler E, Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5 (1) 79-88
    CrossrefPubMedSearch in Google Scholar
  • 4 Lanza R , et al , eds. Handbook of Stem Cells. San Diego, CA: Elsevier/Academic Press; 2004. . Embryonic Stem Cells, vol 1
    Search in Google Scholar
  • 5 Hoffman LM, Carpenter MK. Characterization and culture of human embryonic stem cells. Nat Biotechnol 2005; 23 (6) 699-708
    CrossrefPubMedSearch in Google Scholar
  • 6 Smith AG. Embryo-derived stem cells: of mice and men. Annu Rev Cell Dev Biol 2001; 17: 435-462
    CrossrefPubMedSearch in Google Scholar
  • 7 Passier R, Denning C, Mummery C. Cardiomyocytes from human embryonic stem cells. Handbook Exp Pharmacol 2006; (174) 101-122
    PubMedSearch in Google Scholar
  • 8 Ben-Hur T. Human embryonic stem cells for neuronal repair. Isr Med Assoc J 2006; 8 (2) 122-126 Review
    PubMedSearch in Google Scholar
  • 9 Klimanskaya I, Hipp J, Rezai KA, West M, Atala A, Lanza R. Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics. Cloning Stem Cells 2004; 6 (3) 217-245
    PubMedSearch in Google Scholar
  • 10 Klimanskaya I, Rosenthal N, Lanza R. Derive and conquer: sourcing and differentiating stem cells for therapeutic applications. Nat Rev Drug Discov 2008; 7 (2) 131-142
    CrossrefPubMedSearch in Google Scholar
  • 11 Kroon E, Martinson LA, Kadoya K , et al. Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol 2008; 26 (4) 443-452
    CrossrefPubMedSearch in Google Scholar
  • 12 Schwartz SD, Hubschman JP, Heilwell G , et al. Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet 2012; 379 (9817) 713-720
    CrossrefPubMedSearch in Google Scholar
  • 13 Hug K. Sources of human embryos for stem cell research: ethical problems and their possible solutions. Medicina (Kaunas) 2005; 41 (12) 1002-1010
    PubMedSearch in Google Scholar
  • 14 Hug K. Therapeutic perspectives of human embryonic stem cell research versus the moral status of a human embryo—does one have to be compromised for the other?. Medicina (Kaunas) 2006; 42 (2) 107-114
    PubMedSearch in Google Scholar
  • 15 Evans M. Ethical sourcing of human embryonic stem cells—rational solutions?. Nat Rev Mol Cell Biol 2005; 6 (8) 663-667
    CrossrefPubMedSearch in Google Scholar
  • 16 Lanza RP, Cibelli JB, West MD, Dorff E, Tauer C, Green RM. The ethical reasons for stem cell research. Science 2001; 292 (5520) 1299
    PubMedSearch in Google Scholar
  • 17 McLaren A. A scientist's view of the ethics of human embryonic stem cell research. Cell Stem Cell 2007; 1 (1) 23-26
    CrossrefPubMedSearch in Google Scholar
  • 18 Winston RM. Does government regulation inhibit embryonic stem cell research and can it be effective?. Cell Stem Cell 2007; 1 (1) 27-34
    CrossrefPubMedSearch in Google Scholar
  • 19 Denker HW. Human embryonic stem cells: the real challenge for research as well as for bioethics is still ahead of us. Cells Tissues Organs 2008; 187 (4) 250-256
    CrossrefPubMedSearch in Google Scholar
  • 20 Intemann KK, de Melo-Martín I. Regulating scientific research: should scientists be left alone?. FASEB J 2008; 22 (3) 654-658
    PubMedSearch in Google Scholar
  • 21 The President's Council on Bioethics. White Paper: Alternative Sources of Human Pluripotent Stem Cells. Washington, DC: National Academic Press; 2005
    Search in Google Scholar
  • 22 Hyun I. The bioethics of stem cell research and therapy. J Clin Invest 2010; 120 (1) 71-75
    CrossrefPubMedSearch in Google Scholar
  • 23 Tweedell KS. New paths to pluripotent stem cells. Curr Stem Cell Res Ther 2008; 3 (3) 151-162 Review
    CrossrefPubMedSearch in Google Scholar
  • 24 Cibelli JB, Grant KA, Chapman KB , et al. Parthenogenetic stem cells in nonhuman primates. Science 2002; 295 (5556) 819
    CrossrefPubMedSearch in Google Scholar
  • 25 De Sousa PA, Wilmut I. Human parthenogenetic embryo stem cells: appreciating what you have when you have it. Cell Stem Cell 2007; 1 (3) 243-244
    CrossrefPubMedSearch in Google Scholar
  • 26 Dighe V, Clepper L, Pedersen D , et al. Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotes. Stem Cells 2008; 26 (3) 756-766
    CrossrefPubMedSearch in Google Scholar
  • 27 Mai Q, Yu Y, Li T , et al. Derivation of human embryonic stem cell lines from parthenogenetic blastocysts. Cell Res 2007; 17 (12) 1008-1019
    CrossrefPubMedSearch in Google Scholar
  • 28 Lin G, OuYang Q, Zhou X , et al. A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure. Cell Res 2007; 17 (12) 999-1007
    CrossrefPubMedSearch in Google Scholar
  • 29 Revazova ES, Turovets NA, Kochetkova OD , et al. HLA homozygous stem cell lines derived from human parthenogenetic blastocysts. Cloning Stem Cells 2008; 10 (1) 11-24
    CrossrefPubMedSearch in Google Scholar
  • 30 Brevini TA, Gandolfi F. Parthenotes as a source of embryonic stem cells. Cell Prolif 2008; 41 (Suppl. 01) 20-30
    PubMedSearch in Google Scholar
  • 31 Sung LY, Chang CC, Amano T , et al. Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. Cell Reprogram 2010; 12 (2) 203-211
    CrossrefPubMedSearch in Google Scholar
  • 32 Turovets N, D'Amour KA, Agapov V , et al. Human parthenogenetic stem cells produce enriched populations of definitive endoderm cells after trichostatin A pretreatment. Differentiation 2011; 81 (5) 292-298
    CrossrefPubMedSearch in Google Scholar
  • 33 Isaev DA, Garitaonandia I, Abramihina TV , et al. In vitro differentiation of human parthenogenetic stem cells into neural lineages. Regen Med 2012; 7 (1) 37-45
    CrossrefPubMedSearch in Google Scholar
  • 34 Taranger CK, Noer A, Sørensen AL, Håkelien AM, Boquest AC, Collas P. Induction of dedifferentiation, genomewide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic stem cells. Mol Biol Cell 2005; 16 (12) 5719-5735
    CrossrefPubMedSearch in Google Scholar
  • 35 Håkelien AM, Gaustad KG, Taranger CK, Skålhegg BS, Küntziger T, Collas P. Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression. Exp Cell Res 2005; 309 (1) 32-47
    CrossrefPubMedSearch in Google Scholar
  • 36 Do JT, Schöler HR. Nuclei of embryonic stem cells reprogram somatic cells. Stem Cells 2004; 22 (6) 941-949
    CrossrefPubMedSearch in Google Scholar
  • 37 Tada M, Tada T, Lefebvre L, Barton SC, Surani MA. Embryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells. EMBO J 1997; 16 (21) 6510-6520
    CrossrefPubMedSearch in Google Scholar
  • 38 Tada M, Takahama Y, Abe K, Nakatsuji N, Tada T. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol 2001; 11 (19) 1553-1558
    CrossrefPubMedSearch in Google Scholar
  • 39 Strelchenko N, Kukharenko V, Shkumatov A, Verlinsky O, Kuliev A, Verlinsky Y. Reprogramming of human somatic cells by embryonic stem cell cytoplast. Reprod Biomed Online 2006; 12 (1) 107-111
    CrossrefPubMedSearch in Google Scholar
  • 40 Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006; 126 (4) 663-676
    CrossrefPubMedSearch in Google Scholar
  • 41 Takahashi K, Tanabe K, Ohnuki M , et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007; 131 (5) 861-872
    CrossrefPubMedSearch in Google Scholar
  • 42 Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature 2007; 448 (7151) 313-317
    CrossrefPubMedSearch in Google Scholar
  • 43 Lewitzky M, Yamanaka S. Reprogramming somatic cells towards pluripotency by defined factors. Curr Opin Biotechnol 2007; 18 (5) 467-473 Review
    CrossrefPubMedSearch in Google Scholar
  • 44 Hanna J, Wernig M, Markoulaki S , et al. Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science 2007; 318 (5858) 1920-1923
    CrossrefPubMedSearch in Google Scholar
  • 45 Yu J, Vodyanik MA, Smuga-Otto K , et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318 (5858) 1917-1920
    CrossrefPubMedSearch in Google Scholar
  • 46 Meissner A, Wernig M, Jaenisch R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nat Biotechnol 2007; 25 (10) 1177-1181
    CrossrefPubMedSearch in Google Scholar
  • 47 Jaenisch R, Young R. Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell 2008; 132 (4) 567-582
    CrossrefPubMedSearch in Google Scholar
  • 48 Stadtfeld M, Brennand K, Hochedlinger K. Reprogramming of pancreatic beta cells into induced pluripotent stem cells. Curr Biol 2008; 18 (12) 890-894
    CrossrefPubMedSearch in Google Scholar
  • 49 Hanna J, Markoulaki S, Schorderet P , et al. Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency. Cell 2008; 133 (2) 250-264
    CrossrefPubMedSearch in Google Scholar
  • 50 Park IH, Zhao R, West JA , et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008; 451 (7175) 141-146
    CrossrefPubMedSearch in Google Scholar
  • 51 Park IH, Zhao R, West JA , et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008; 451 (7175) 141-146
    CrossrefPubMedSearch in Google Scholar
  • 52 Lowry WE, Richter L, Yachechko R , et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A 2008; 105 (8) 2883-2888
    CrossrefPubMedSearch in Google Scholar
  • 53 Brambrink T, Foreman R, Welstead GG , et al. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2008; 2 (2) 151-159
    CrossrefPubMedSearch in Google Scholar
  • 54 Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell 2008; 2 (3) 230-240
    CrossrefPubMedSearch in Google Scholar
  • 55 Wernig M, Zhao JP, Pruszak J , et al. Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. Proc Natl Acad Sci U S A 2008; 105 (15) 5856-5861
    CrossrefPubMedSearch in Google Scholar
  • 56 Schenke-Layland K, Rhodes KE, Angelis E , et al. Reprogrammed mouse fibroblasts differentiate into cells of the cardiovascular and hematopoietic lineages. Stem Cells 2008; 26 (6) 1537-1546
    CrossrefPubMedSearch in Google Scholar
  • 57 Yu J, Vodyanik MA, Smuga-Otto K , et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318 (5858) 1917-1920
    CrossrefPubMedSearch in Google Scholar
  • 58 Shi Y, Do JT, Desponts C, Hahm HS, Schöler HR, Ding S. A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2008; 2 (6) 525-528
    CrossrefPubMedSearch in Google Scholar
  • 59 Duinsbergen D, Salvatori D, Eriksson M, Mikkers H. Tumors originating from induced pluripotent stem cells and methods for their prevention. Ann N Y Acad Sci 2009; 1176: 197-204
    CrossrefPubMedSearch in Google Scholar
  • 60 Miura K, Okada Y, Aoi T , et al. Variation in the safety of induced pluripotent stem cell lines. Nat Biotechnol 2009; 27 (8) 743-745
    CrossrefPubMedSearch in Google Scholar
  • 61 Nakagawa M, Koyanagi M, Tanabe K , et al. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 2008; 26 (1) 101-106
    CrossrefPubMedSearch in Google Scholar
  • 62 Meng X, Neises A, Su RJ , et al. Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Mol Ther 2012; 20 (2) 408-416
    CrossrefPubMedSearch in Google Scholar
  • 63 Liu T, Zou G, Gao Y , et al. High efficiency of reprogramming CD34+ cells derived from human amniotic fluid into induced pluripotent stem cells with Oct4. Stem Cells Dev 2012; 21 (12) 2322-2332
    CrossrefPubMedSearch in Google Scholar
  • 64 Zhao HX, Li Y, Jin HF , et al. Rapid and efficient reprogramming of human amnion-derived cells into pluripotency by three factors OCT4/SOX2/NANOG. Differentiation 2010; 80 (2–3) 123-129
    CrossrefPubMedSearch in Google Scholar
  • 65 Tsai SY, Clavel C, Kim S , et al. Oct4 and klf4 reprogram dermal papilla cells into induced pluripotent stem cells. Stem Cells 2010; 28 (2) 221-228
    PubMedSearch in Google Scholar
  • 66 Wei Z, Yang Y, Zhang P , et al. Klf4 interacts directly with Oct4 and Sox2 to promote reprogramming. Stem Cells 2009; 27 (12) 2969-2978
    PubMedSearch in Google Scholar
  • 67 Kim JB, Greber B, Araúzo-Bravo MJ , et al. Direct reprogramming of human neural stem cells by OCT4. Nature 2009; 461 (7264) 649-3
    CrossrefPubMedSearch in Google Scholar
  • 68 Miyazaki S, Yamamoto H, Miyoshi N, Takahashi H, Suzuki Y, Haraguchi N, Ishii H, Doki Y, Mori M. Emerging methods for preparing iPS cells. Jpn J Clin Oncol 2012; 42 (9) 773-779
    CrossrefPubMedSearch in Google Scholar
  • 69 Yu J, Hu K, Smuga-Otto K , et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 2009; 324 (5928) 797-801
    CrossrefPubMedSearch in Google Scholar
  • 70 Woltjen K, Michael IP, Mohseni P , et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 2009; 458 (7239) 766-770
    CrossrefPubMedSearch in Google Scholar
  • 71 Rhee YH, Ko JY, Chang MY , et al. Protein-based human iPS cells efficiently generate functional dopamine neurons and can treat a rat model of Parkinson disease. J Clin Invest 2011; 121 (6) 2326-2335
    CrossrefPubMedSearch in Google Scholar
  • 72 Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 2009; 458 (7239) 771-775
    CrossrefPubMedSearch in Google Scholar
  • 73 Hiratsuka M, Uno N, Ueda K , et al. Integration-free iPS cells engineered using human artificial chromosome vectors. PLoS ONE 2011; 6 (10) e25961
    CrossrefPubMedSearch in Google Scholar
  • 74 Okita K, Matsumura Y, Sato Y , et al. A more efficient method to generate integration-free human iPS cells. Nat Methods 2011; 8 (5) 409-412
    CrossrefPubMedSearch in Google Scholar
  • 75 Yu J, Chau KF, Vodyanik MA, Jiang J, Jiang Y. Efficient feeder-free episomal reprogramming with small molecules. PLoS ONE 2011; 6 (3) e17557
    CrossrefPubMedSearch in Google Scholar
  • 76 Hu K, Yu J, Suknuntha K , et al. Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells. Blood 2011; 117 (14) e109-e119
    CrossrefPubMedSearch in Google Scholar
  • 77 Yu J, Hu K, Smuga-Otto K , et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 2009; 324 (5928) 797-801
    CrossrefPubMedSearch in Google Scholar
  • 78 Banito A, Rashid ST, Acosta JC , et al. Senescence impairs successful reprogramming to pluripotent stem cells. Genes Dev 2009; 23 (18) 2134-2139
    CrossrefPubMedSearch in Google Scholar
  • 79 Feng Q, Lu SJ, Klimanskaya I , et al. Hemangioblastic derivatives from human induced pluripotent stem cells exhibit limited expansion and early senescence. Stem Cells 2010; 28 (4) 704-712
    CrossrefPubMedSearch in Google Scholar
  • 80 Mayshar Y, Ben-David U, Lavon N , et al. Identification and classification of chromosomal aberrations in human induced pluripotent stem cells. Cell Stem Cell 2010; 7 (4) 521-531
    CrossrefPubMedSearch in Google Scholar
  • 81 Lister R, Pelizzola M, Kida YS , et al. Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells. Nature 2011; 471 (7336) 68-73
    CrossrefPubMedSearch in Google Scholar
  • 82 Laurent LC, Ulitsky I, Slavin I , et al. Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in culture. Cell Stem Cell 2011; 8 (1) 106-118
    CrossrefPubMedSearch in Google Scholar
  • 83 Gore A, Li Z, Fung HL , et al. Somatic coding mutations in human induced pluripotent stem cells. Nature 2011; 471 (7336) 63-67
    CrossrefPubMedSearch in Google Scholar
  • 84 Chung Y, Klimanskaya I, Becker S , et al. Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres. Nature 2006; 439 (7073) 216-219
    CrossrefPubMedSearch in Google Scholar
  • 85 Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R. Human embryonic stem cell lines derived from single blastomeres [published correction appears in Nature 2006;444(7118):512, and Nature 2007;446(7133):342]. Nature 2006; 444 (7118) 481-485
    CrossrefPubMedSearch in Google Scholar
  • 86 Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R. Derivation of human embryonic stem cells from single blastomeres. Nat Protoc 2007; 2 (8) 1963-1972
    CrossrefPubMedSearch in Google Scholar
  • 87 Chung Y, Klimanskaya I, Becker S , et al. Human embryonic stem cell lines generated without embryo destruction. Cell Stem Cell 2008; 2 (2) 113-117
    CrossrefPubMedSearch in Google Scholar
  • 88 Handyside AH, Kontogianni EH, Hardy K, Winston RM. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature 1990; 344 (6268) 768-770
    CrossrefPubMedSearch in Google Scholar
  • 89 Kelly SJ. Studies of the developmental potential of 4- and 8-cell stage mouse blastomeres. J Exp Zool 1977; 200 (3) 365-376
    CrossrefPubMedSearch in Google Scholar
  • 90 Pedersen RA. Potency, lineage and allocation in preimplantation mouse embryos. In: Rossant J, Pedersen RA, , eds. Experimental Approaches to Mammalian Embryonic Development. New York, NY: Cambridge University Press; 1986: 3-33
    Search in Google Scholar
  • 91 Piotrowska K, Wianny F, Pedersen RA, Zernicka-Goetz M. Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development. Development 2001; 128 (19) 3739-3748
    PubMedSearch in Google Scholar
  • 92 Piotrowska-Nitsche K, Zernicka-Goetz M. Spatial arrangement of individual 4-cell stage blastomeres and the order in which they are generated correlate with blastocyst pattern in the mouse embryo. Mech Dev 2005; 122 (4) 487-500
    CrossrefPubMedSearch in Google Scholar
  • 93 Piotrowska-Nitsche K, Perea-Gomez A, Haraguchi S, Zernicka-Goetz M. Four-cell stage mouse blastomeres have different developmental properties. Development 2005; 132 (3) 479-490
    CrossrefPubMedSearch in Google Scholar
  • 94 Lorthongpanich C, Yang SH, Piotrowska-Nitsche K, Parnpai R, Chan AW. Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells. Reproduction 2008; 135 (6) 805-813
    CrossrefPubMedSearch in Google Scholar
  • 95 Teramura T, Takehara T, Kishi N , et al. A mouse and embryonic stem cell derived from a single embryo. Cloning Stem Cells 2007; 9 (4) 485-494
    CrossrefPubMedSearch in Google Scholar
  • 96 González S, Ibáñez E, Santaló J. Establishment of mouse embryonic stem cells from isolated blastomeres and whole embryos using three derivation methods. J Assist Reprod Genet 2010; 27 (12) 671-682
    CrossrefPubMedSearch in Google Scholar
  • 97 Lorthongpanich C, Yang SH, Piotrowska-Nitsche K, Parnpai R, Chan AW. Development of single mouse blastomeres into blastocysts, outgrowths and the establishment of embryonic stem cells. Reproduction 2008; 135 (6) 805-813
    CrossrefPubMedSearch in Google Scholar
  • 98 Wakayama S, Hikichi T, Suetsugu R , et al. Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies. Stem Cells 2007; 25 (4) 986-993
    CrossrefPubMedSearch in Google Scholar
  • 99 Fong CY, Richards M, Bongso A. Unsuccessful derivation of human embryonic stem cell lines from pairs of human blastomeres. Reprod Biomed Online 2006; 13 (2) 295-300
    CrossrefPubMedSearch in Google Scholar
  • 100 Johnson MH, McConnell JM. Lineage allocation and cell polarity during mouse embryogenesis. Semin Cell Dev Biol 2004; 15 (5) 583-597
    CrossrefPubMedSearch in Google Scholar
  • 101 Fleming TP, Papenbrock T, Fesenko I, Hausen P, Sheth B. Assembly of tight junctions during early vertebrate development. Semin Cell Dev Biol 2000; 11 (4) 291-299
    PubMedSearch in Google Scholar
  • 102 Rossant J. Lineage development and polar asymmetries in the peri-implantation mouse blastocyst. Semin Cell Dev Biol 2004; 15 (5) 573-581
    PubMedSearch in Google Scholar
  • 103 Johnson MH, Ziomek CA. The foundation of two distinct cell lineages within the mouse morula. Cell 1981; 24 (1) 71-80
    CrossrefPubMedSearch in Google Scholar
  • 104 Krtolica A, Genbacev O, Escobedo C , et al. Disruption of apical-basal polarity of human embryonic stem cells enhances hematoendothelial differentiation. Stem Cells 2007; 25 (9) 2215-2223
    CrossrefPubMedSearch in Google Scholar
  • 105 Cowan CA, Klimanskaya I, McMahon J , et al. Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med 2004; 350 (13) 1353-1356
    CrossrefPubMedSearch in Google Scholar
  • 106 Klimanskaya I, McMahon J. Approaches for derivation and maintenance of human embryonic stem cells. Detailed procedures and alternatives. In: Lanza R, Hogan B, Melton D, Pedersen R, Thomson J, , eds. Essentials of Stem Cell Biology. Amsterdam, the Netherlands: Elsevier Science; 2005: 287-304
    Search in Google Scholar
  • 107 Ilic D, Giritharan G, Zdravkovic T , et al. Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. Stem Cells Dev 2009; 18 (9) 1343-1350
    CrossrefPubMedSearch in Google Scholar
  • 108 Feki A, Bosman A, Dubuisson JB , et al. Derivation of the first Swiss human embryonic stem cell line from a single blastomere of an arrested four-cell stage embryo. Swiss Med Wkly 2008; 138 (37–38) 540-550
    PubMedSearch in Google Scholar
  • 109 Geens M, Mateizel I, Sermon K , et al. Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos. Hum Reprod 2009; 24 (11) 2709-2717
    CrossrefPubMedSearch in Google Scholar
  • 110 Giritharan G, Ilic D, Gormley M, Krtolica A. Human embryonic stem cells derived from embryos at different stages of development share similar transcription profiles. PLoS ONE 2011; 6 (10) e26570
    CrossrefPubMedSearch in Google Scholar
  • 111 Lu B, Malcuit C, Wang S , et al. Long-term safety and function of RPE from human embryonic stem cells in preclinical models of macular degeneration. Stem Cells 2009; 27 (9) 2126-2135
    CrossrefPubMedSearch in Google Scholar