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Semin Reprod Med 2015; 33(06): 410-421
DOI: 10.1055/s-0035-1567826
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Autologous Germline Mitochondrial Energy Transfer (AUGMENT) in Human Assisted Reproduction

Dori C. Woods
1   Laboratory of Aging and Infertility Research (LAIR), Department of Biology, Northeastern University, Boston, Massachusetts
,
Jonathan L. Tilly
1   Laboratory of Aging and Infertility Research (LAIR), Department of Biology, Northeastern University, Boston, Massachusetts
2   Office of the Dean, College of Science, Northeastern University, Boston, Massachusetts
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Publication History

Publication Date:
17 November 2015 (online)

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Abstract

Ovarian aging is characterized by a decline in both the total number and overall quality of oocytes, the latter of which has been experimentally tied to mitochondrial dysfunction. Clinical studies in the late 1990s demonstrated that transfer of cytoplasm aspirated from eggs of young female donors into eggs of infertile women at the time of intracytoplasmic sperm injection improved pregnancy success rates. However, donor mitochondria were identified in offspring, and the United States Food and Drug Administration raised questions about delivery of foreign genetic material into human eggs at the time of fertilization. Accordingly, heterologous cytoplasmic transfer, while promising, was in effect shut down as a clinical protocol. The recent discovery of adult oogonial (oocyte-generating) stem cells in mice, and subsequently in women, has since re-opened the prospects of delivering a rich source of pristine and patient-matched germline mitochondria to boost egg health and embryonic developmental potential without the need for young donor eggs to obtain cytoplasm. Herein we overview the science behind this new protocol, which has been patented and termed autologous germline mitochondrial energy transfer, and its use to date in clinical studies for improving pregnancy success in women with a prior history of assisted reproduction failure.

Keywords

germline stem cell - oogonial stem cell - mitochondria - ooplasmic transfer - oocyte - in vitro fertilization

Note

During the final preparation of this article, additional key studies of central importance to verifying the ability of isolated mouse OSCs to generate fertilization competent eggs and viable offspring,[97] and to identifying the existence of OSCs in adult baboon ovaries,[98] were published; additionally, a third clinical site reporting its experience with AUGMENT in human assisted reproduction was published.[99]