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Thromb Haemost 1994; 71(05): 596-604
DOI: 10.1055/s-0038-1642489
Review Article
Schattauer GmbH Stuttgart

Phospholipid-Specific Conformational Changes in Human Prothrombin upon Binding to Procoagulant Acidic Lipid Membranes[*]

Jogin R Wu
The Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
,
Barry R Lentz
The Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
› Author Affiliations
Further Information

Publication History

Received 08 September 1993

Accepted after revision 11 January 1994

Publication Date:
06 July 2018 (online)

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Summary

This paper provides evidence to demonstrate that human prothrombin undergoes conformational changes upon binding to procoagulant membranes specifically containing phosphatidylserine (PS). Fourier transform infrared spectroscopy was used to show a slight increase in ordered (α-helix, β-sheet, β-turns) secondary structure upon binding to PS-containing membranes. Thermograms representing prothrombin and prothrombin fragment 1 denaturation were obtained using differential scanning calorimetry. These were analyzed and interpreted in terms of changes in prothrombin domain organization associated with binding to PS-containing membranes. Changes in either secondary structure or domain organization upon binding to negatively-charged phosphatidylglycerol-containing membranes were, if they occurred at all, much less dramatic. The results paralleled results obtained previously with bovine prothrombin (1, 2). The implications of these results in terms of a possible molecular mechanism for the cofactor-like role of platelet membrane vesicles in prothrombin activation are discussed.

Notes

Supported by USPHS Grant HL45916


 

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