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Thromb Haemost 1986; 55(02): 276-278
DOI: 10.1055/s-0038-1642536
Original Article
Schattauer GmbH Stuttgart

Visualization of von Willebrand Factor Multimers by Enzyme-Conjugated Secondary Antibodies

F Brosstad
The Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway
,
Inge Kjønniksen
The Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway
,
B Rønning
The Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway
,
H Stormorken
The Research Institute for Internal Medicine, Rikshospitalet, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received 25 November 1985

Accepted 21 February 1986

Publication Date:
18 July 2018 (online)

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Summary

A method for visualization of the multimeric forms of von Willebrand Factor (vWF) in plasma and platelets is described. The method is based upon: 1) Separation of the vWF multimers by SDS-agarose electrophoresis, 2) Subsequent blotting of the vWF multimers onto nitrocellulose, 3) Immunolocalization and visualization of the vWF pattern by the sequential incubation of the blot with a) primary vWF antiserum, b) peroxidase- or beta-galactosidase-conjugated secondary antibodies and a relevant chromogenic substrate.

Key words

Von Willebrand factor (vWF) - Thrombus formation - Multimers - Visualization - Enzyme-linked antibodies
 

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