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Thromb Haemost 1988; 59(02): 231-235
DOI: 10.1055/s-0038-1642760
Original Articles
Schattauer GmbH Stuttgart

Monocytes of Patients Congenitally Deficient in Plasma Factor XIII Lack Factor XIII Subunit A Antigen and Transglutaminase Activity

László Muszbek
1   The Department of Clinical Chemistry, University School of Medicine, Debrecen, Hungary
,
Roza Ádány
1   The Department of Clinical Chemistry, University School of Medicine, Debrecen, Hungary
,
Mária Kávai
2   IIIrd Department of Medicine, University School of Medicine, Debrecen, Hungary
,
Zoltán Boda
3   IInd Department of Medicine, University School of Medicine, Debrecen, Hungary
,
Stanislaw Lopaciuk
4   Institute of Haematology, Warsaw, Poland
› Author Affiliations
Further Information

Publication History

Received 12 October 1987

Accepted after revision 27 November 1987

Publication Date:
21 May 2018 (online)

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Summary

Monocytes isolated from patients with severe deficiency in plasma Factor XIII of blood coagulation (FXIII) were tested for FXIII antigen and transglutaminase activity. By immunoperoxi- dase method the patients’ monocytes, in contrast to normal controls, showed no reaction with a monospecific antibody against FXIII subunit ª.This result was confirmed by immuno- blotting technique, as well. In addition, tissue macrophages tested in one of the patients were also exempt of FXIII subunit ª antigen. The transglutaminase activity in FXIII deficient monocytes was below the limit of the detection of the dansylcadaverine incorporation assay. The results suggest that FXIII subunitªof monocytes/macrophages and its plasma and platelet counterparts are closely related or identical proteins and demonstrate that the transglutaminase activity in monocytes is of FXIII origin and tissue transglutaminase is present, if at all, only in insignificant amount.

Keywords

Factor XIII - Monocytes - Transglutaminase
 

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