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Thromb Haemost 1989; 61(01): 025-029
DOI: 10.1055/s-0038-1646521
Original Article
Schattauer GmbH Stuttgart

The Effect of Trace Amounts of Tissue Factor on Thrombin Generation in Platelet Rich Plasma, its Inhibition by Heparin

S Béguin
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
,
T Lindhout
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
,
H C Hemker
The Department of Biochemistry, University of Limburg, Maastricht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 08 March 1988

Accepted after revision 21 September 1988

Publication Date:
24 July 2018 (online)

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Summary

Amounts of human brain thromboplastin that do not stimulate thrombin generation in platelet poor plasma, were shown to advance by about 4 min an explosive formation of thrombin that occurs after recalcification in the presence of blood platelets. This synergistic effect is inhibited by the specific thrombin inhibitor hirudin and mimicked by adding low concentrations (< 5 nM) of thrombin to platelet rich plasma. It is our conclusion that, small amounts of thrombin, generated under the influence of thromboplastin induced procoagulant activity in the blood platelets. This activity is most likely mainly due to procoagulant phospholipids. Heparin inhibits this effect and retards the explosive thrombin formation. It does not, however, diminish the peak amount of thrombin eventually formed, because heparin neutralizing material released from the activated platelets quenches the heparin effect.

Key words

Platelets - Tissue factor - Thrombin - Heparin
 

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