The Effect of Va rious Anticoagulant/Anti platelet Mixtures on Determination of Plasminogen Activator Inhibitor, Platelet Proteins and Hemostasis Farameters

E Eriksson; L Lengborn; B Risberg

doi:10.1055/s-0038-1646625

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1989; 61(03): 511-516
DOI: 10.1055/s-0038-1646625

Original Article

Schattauer GmbH Stuttgart

The Effect of Va rious Anticoagulant/Anti platelet Mixtures on Determination of Plasminogen Activator Inhibitor, Platelet Proteins and Hemostasis Farameters

E Eriksson

Östra and Sahlgrenska Hospitals, University of Göteborg, Sweden

,

L Lengborn

^*The Departments of Surgery I and Medicine II, University of Göteborg, Sweden

Östra and Sahlgrenska Hospitals, University of Göteborg, Sweden

,

B Risberg

Östra and Sahlgrenska Hospitals, University of Göteborg, Sweden

› Author Affiliations

Abstract

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Summary

Blood collected in different anticoagulant/antiplatelet agents(ETP, EDTA, citrate, citratelcitric acid pH 4.5 and CTAD) was compared with respect to determination of PAI-1 activity and PAI-1 antigen. βTG and PF4 were analysed as markers of platelet release. Both the middle layer and the remaining layer of the plasma were studied. Moreover vWF:Ag, FVII:Ag, ECLI t-PA:Ag, t-PA activity, APTT VIII: C and VII: C were assayed in blood collected in citrate and CTAD

PAI-1 activity showed the same level in all citrate based anticoagulants and ETP and no increase was found in blood standing for 2 hours at room temperature. On the contrary quick handling was most important for determination of PAI-1 antigen. In tubes anticoagulated with citrate no significant increase was found if the sample was prepared within 1 hour. EDTA was not suitable as anticoagulant mixture. Tubes containing the antiplatelet mixture CTAD could be used for determination of PAI activity, PAI antigen, vWF:Ag, FVIIiAg, t-PA activity and APTT.

For measurement of PAI-1 antigen quick handling of blood anticoagulated with antiplatelet mixtures are preferable, and plasma treated in that manner could also be used to assay some hemostasis parameters.

Keywords

Blood sampling - Plasminogen activator inhibitor - Platelets - β-Thromboglobulin - Platelet factor 4

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References

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