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Thromb Haemost 1988; 60(03): 457-462
DOI: 10.1055/s-0038-1646990
Original Article
Schattauer GmbH Stuttgart

The Mode of Action of Heparin in Plasma[*]

Suzette Béguin
The Department of Biochemistry, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
Theo Lindhout
The Department of Biochemistry, Biomedical Center, University of Limburg, Maastricht, The Netherlands
,
H Coenraad Hemker
The Department of Biochemistry, Biomedical Center, University of Limburg, Maastricht, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 20 January 1988

Accepted after revision 05 August 1988

Publication Date:
23 July 2018 (online)

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Summary

The influence of heparin on prothrombin conversion in plasma was estimated by measuring prothrombin disappearance with the aid of staphylocoagulase or by calculation from the thrombin generation curve, compensating for simultaneous thrombin inactivation by plasma protease inhibitors. In thromboplastin-activated plasma the effect of heparin on prothrombinase (factor Xa-factor Va-phospholipid) is negligible compared to that on thrombin probably because both the extrinsic factor X activating complex and phospholipid bound factor Xa are insensitive to AT III-heparin action. In contact-activated plasma prothrombinase generation is inhibited by heparin, because heparin lowers the ambient concentrations of thrombin so that the feedback activation of factor VIII by thrombin is diminished. Consequently, the delayed factor Xa generation causes a lag phase in prothrombinase generation. We conclude that heparin in plasma acts by its thrombin scavenging properties. No direct action on prothrombinase or on the factor X activating enzyme needs to be assumed if one takes into account the effect of thrombin depletion on thrombin-mediated feedback reactions

Keywords

Prothrombin activation - Heparin - Plasma protease inhibitors

Part of this work has been presented at the XIth International Society of Thrombosis and Haemostasis Congress, Brussels, 1987


 

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