Summary
Monoclonal antibodies to human fibrin have been prepared from stable hybridomas, obtained
by fusion of a mouse myeloma cell line (NS-1) and spleen cells of Balb/c mice immunized
with a suspension of human fibrin. One cell line, DG1, producing a monoclonal antibody
of the IgG1 κ subclass, reacted specifically with human fibrin (KD = 1.2 nM). Western blotting analysis indicates that DG1 crossreacts with the fibrin
fragment D-dimer. Using both a chromogenic and an 125I-fibrin release assay it was illustrated that in the presence of the fibrin specific
antibody the t-PA mediated generation of plasmin was significantly inhibited.
An animal model system, developed to monitor thrombosis and induced reactive fibrinolysis,
was used to investigate the interference of plasminogen activation, by the antibody,
in vivo.
This fibrin specific antibody prolonged the onset of reactive fibrinolysis in a dose
dependent manner.
Keywords
Fibrin specific antibody - Tissue plasminogen activator
