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Thromb Haemost 1988; 59(03): 435-439
DOI: 10.1055/s-0038-1647511
Original Article
Schattauer GmbH Stuttgart

Aggregation of Human Platelets by Acidic Mucopolysaccharide Extracted from Stichopus japonicus Selenka

Jia-Zeng Li
*   Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin, China
,
Eric Chun-Yet Lian
The Veterans Administration Medical Center, Hemophilia and Thrombosis Center, Center for Blood Diseases, University of Miami, Miami, Florida, USA
› Author Affiliations
Further Information

Publication History

Received 06 May 1987

Accepted after revision 04 February 1988

Publication Date:
29 June 2018 (online)

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Summary

The acidic mucopolysaccharide extracted from sea cucumber (Stichopus japonicus Selenka) (SJAMP) has been shown to cause platelets to aggregate. Using citrated platelet-rich plasma (PRP), washed platelets and formaldehyde-fixed platelets from humans, we investigated the effects of platelet inhibitors and various plasmas and their fractions on SJAMP-induced platelet aggregation. It was found that the lowest concentration of SJAMP required for the aggregation of human platelets was about 0.4 μg/ ml and the magnitude of aggregation induced by SJAMP was concentration dependent. The platelets were aggregated by SJAMP at 10 μg/ml in 25 out of 28 (89%) normal subjects tested. Platelet inhibitors such as PGE1, aspirin, indomethacin, apyrase, antimycin, 2-deoxy-D-glucose and EDTA inhibited by 70 to 100% the aggregation induced by SJAMP. Washed platelets alone were not aggregated by SJAMP. In the presence of fibrinogetr, washed platelets were aggregated by SJAMP, but formaldehyde-fixed platelets were not. These data indicate that the SJAMP-induced human platelet aggregation requires extracellular calcium, fibrinogen, and energy metabolism. The second phase of aggregation is dependent upon the release of ADP, and cyclooxygenase pathway.

Keywords

Platelet aggregation - Acidic mucopolysaccharide - Humans
 

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