Induction of Tissue Factor Synthesis in Human Umbilical Vein Endothelial Cells Involves Protein Kinase C

Summary

Incubation of human umbilical vein endothelial cells with one of the following compounds: endotoxin, recombinant interleukin-1β, recombinant tumor necrosis factor α, allogenic lymphocyte subpopulations or phorbol ester resulted in significant induction of tissue factor synthesis. Diacylglycerol had the same effect and also enhanced synergistically the induction caused by endotoxin and interleukin-1β. Two different inhibitors of protein kinase C, H7 and sphingosine, inhibited tissue factor synthesis at concentrations which did not depress protein synthesis in general, suggesting that protein kinase C is involved in the processes leading to tissue factor synthesis. Cells down-regulated for the tissue factor response to TPA responded essentially normally to endotoxin and interleukin-1 with regard to tissue factor synthesis.

• References

• 1 Lyberg T, Gaidai KS, Prydz H. Synthesis of thromboplastin protein in endothelial cells. 2nd International Symposium on The Biology of Vascular Endothelial cells. Cambridge, UK: 1981. p 47
• 2 Bevilaqua M, Pober J, Majeau G, Cotran R, Gimbrone M. Interleukin 1 (IL-1) induces biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells. J Exp Med 1984; 160: 618-623
• 3 Bevilaqua M, Pober J, Majeau G, Fiers W, Cotran R, Gimbrone M. Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium; characterization and comparison with the action of interleukin 1. Proc Natl Acad Sci USA 1986; 83: 4533-4537
• 4 Carlsen E, Gaudernack G, Filion-Myklebust C, Pettersen KS, Prydz H. Allogeneic induction of thromboplastin synthesis in monocytes and endothelial cells. Biphasic effect of cyclosporin A. Clin Exp Immunol 1989; 76: 428-433
• 5 Chargaff E, Bendich A, Cohen S. The thromboplastic protein; structure, properties, disintegration. J Biol Chem 1944; 156: 161-178
• 6 Spicer EK, Horton R, Bloem L, Bach R, Williams KR, Guha A, Kraus J, Lin TC, Nemerson Y, Koningsberg WH. Isolation of cDNA clones coding for human tissue factor; primary structure of the protein and cDNA. Proc Natl Acad Sci USA 1987; 84: 5148-5152
• 7 Scarpati EM, Wen D, Broze GJ, Miletich JP, Flandermeyer RR. Human tissue factor: cDNA sequence and chromosome localization of the gene. Biochemistry 1987; 26: 5234-5238
• 8 Fisher K, Gorman CM, Vehar GA, O’Brien DP, Lawn RM. Cloning and expression of human tissue factor cDNA. Thromb Res 1987; 48: 89-99
• 9 Morrissey JH, Fakhrai H, Edgington TS. Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell 1987; 50: 129-135
• 10 Mackman N, Morrissey JH, Fowler B, Edgington TS. Complete sequence of the human tissue factor gene, a highly regulated cellular receptor that initiates the coagulation protease cascade. Biochemistry 1989; 28: 1755-1762
• 11 Bjorklid E, Storm E, Prydz H. The protein component of human brain thromboplastin. Biochem Biophys Res Commun 1973; 55: 969-976
• 12 Bjorklid E, Storm E. Purification and some properties of the protein component of tissue thromboplastin from human brain. Biochem J 1977; 165: 89-96
• 13 Andoh K, Pettersen KS, Filion-Myklebust C, Prydz H. Observation on the cell biology of tissue factor in endothelial cells. Thromb Haemostas 1990; 63: 298-302
• 14 Lyberg T. Intercellular signal mechanisms in induction of thromboplastin synthesis. Haemostasis 1984; 14: 393-399
• 15 Lyberg T, Prydz H. The role of Ca²⁺ in the regulation of thromboplas tin synthesis in human monocytes. In: Lyberg T. Studies on the Cell Biology of Thromboplastin. Ph. D. Thesis, University of Oslo; Oslo, Norway: 1984
• 16 Lyberg T. Effects of cyclic AMP and cyclic GMP on thromboplastin (factor III) synthesis in human monocytes in vitro. Thromb Haemostas 1983; 50: 804-809
• 17 Edgington TS, Pizzolato MC. Regulatory effects of prostaglandins in the T cell instructed monocyte procoagulant response. Thromb Haemostas 1983; 50: 148
• 18 Crutchley DJ. Effects of inhibitors of arachidonic acid metabolism of thromboplastin activity in human monocytes. Biochem Biophys Res Commun 1984; 119: 179-184
• 19 Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, Nishizuka Y. Direct activation of calcium activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem 1982; 257: 7847-7851
• 20 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. J Clin Invest 1973; 52: 2745-2756
• 21 The Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. Recommended methods for the determination of four enzymes in blood. Scand J Clin Lab Invest 1974; 33: 291-306
• 22 Hansen JA, Martin PJ, Beatty PG, Clark EA, Ledbetter JA. Human T lymphocyte cell surface molecules defined by the workshop monoclonal antibodies (“T cell protocol”). In: Leucocyte Typing: Human Leucocyte Markers Detected by Monoclonal Antibodies. Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF. (eds) Springer Verlag; New York: 1984
• 23 Gaudernack G, Leivestad T, Ugelstad J, Thorsby E. Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies directly conjugated to monosized magnetic microspheres. J Immunol Methods 1986; 90: 179-187
• 24 Hvatum M, Prydz H. Studies on tissue thromboplastin. Solubilization with sodium deoxycholate. Biochim Biophys Acta 1966; 130: 92-101
• 25 Markwell MAK, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87: 206-210
• 26 Lyberg T, Gaidai KS, Evensen SA, Prydz H. Cellular cooperation in endothelial cell thromboplastin synthesis. Br J Haematol 1983; 53: 85-95
• 27 Carlsen E, Flatmark A, Prydz H. Cytokine-induced procoagulant activity in monocytes and endothelial cells. Transplantation 1988; 46: 575-580
• 28 Hidaka H, Hagiwara M. Pharmacology of the isoquinoline sulfonamide protein kinase C inhibitors. Trends Pharmacol Sci 1987; 8: 29-32
• 29 Bell RM. Protein kinase activation by diacylglycerol second messengers. Cell 1989; 45: 631-632
• 30 Concling PR, Patton KL, Hannun YA, Greenberg CS, Brice Weinberg J. Sphingosine inhibits monocyte tissue factor-initiated coagulation by altering factor VII binding. J Biol Chem 1989; 264: 18440-18444
• 31 Crossman DC, Carr DP, Tuddenham EGD, Pearson JD, McVey JH. The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester. J Biol Chem 1990; 265: 9782-9787
• 32 Rosen A, Nairn AC, Greengard P, Cohn ZA, Aderem A. Bacterial lipopolysaccharide regulates the phosphorylation of the 68K protein kinase C substrate in macrophages. J Biol Chem 1989; 264: 9118-9121
• 33 Berrebi G, Takayama H, Sitovsky MV. Antigen receptor interaction requirement for conjugate formation and lethal-hit triggering by cytotoxic T lymphocytes can be bypassed by protein kinase C activators and Ca²⁺-ionophores. Proc Natl Acad Sci 1987; 84: 1364-1387
• 34 Russel JH, McCulley DE, Taylor AS. Antagonistic effects of phorbol esters on lymphocyte activation. J Biol Chem 1986; 261: 12643-12648
• 35 Chow SC, Ng J, Nordstedt C, Fredholm BB, Jondal M. Phos-phoinositide breakdown and evidence for protein kinase C involvement during human NK killing. Cell Immunol 1988; 114: 96-103
• 36 Kvanta A, Nordstedt C, Fredholm B, Gullberg M, Jondal M. Evidence that protein kinase C activation is essential for killing by II-2-activated lymphocytes. Scand J Immunol 1990; 31: 103-108