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Thromb Haemost 1992; 67(04): 473-477
DOI: 10.1055/s-0038-1648473
Original Articles
Schattauer GmbH Stuttgart

Induction of Tissue Factor Synthesis in Human Umbilical Vein Endothelial Cells Involves Protein Kinase C

Kjell Sverre Pettersen
The Biotechnology Centre of Oslo, University of Oslo, Rikshospitalet, Oslo, Norway
,
Merete Thune Wiiger
The Biotechnology Centre of Oslo, University of Oslo, Rikshospitalet, Oslo, Norway
,
Nobuhiro Narahara
The Biotechnology Centre of Oslo, University of Oslo, Rikshospitalet, Oslo, Norway
,
Kiyoshi Andoh
The Biotechnology Centre of Oslo, University of Oslo, Rikshospitalet, Oslo, Norway
,
Gustav Gaudernack
1   Institute of Transplantation Immunology, University of Oslo, Rikshospitalet, Oslo, Norway
,
Hans Prydz
The Biotechnology Centre of Oslo, University of Oslo, Rikshospitalet, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received 25 September 1990

Accepted after revision 29 October 1991

Publication Date:
03 July 2018 (online)

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Summary

Incubation of human umbilical vein endothelial cells with one of the following compounds: endotoxin, recombinant interleukin-1β, recombinant tumor necrosis factor α, allogenic lymphocyte subpopulations or phorbol ester resulted in significant induction of tissue factor synthesis. Diacylglycerol had the same effect and also enhanced synergistically the induction caused by endotoxin and interleukin-1β. Two different inhibitors of protein kinase C, H7 and sphingosine, inhibited tissue factor synthesis at concentrations which did not depress protein synthesis in general, suggesting that protein kinase C is involved in the processes leading to tissue factor synthesis. Cells down-regulated for the tissue factor response to TPA responded essentially normally to endotoxin and interleukin-1 with regard to tissue factor synthesis.

 

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